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GFP and Epitopes  >  Antibodies to GST
 

GST Antibody Dylight™ 488 Conjugated

Rabbit Polyclonal
Code

600-441-200
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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DyLight™ dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image.  DyLight™ dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods.  This image shows anti-histone detection using a DyLight™ 488 conjugate (green).  Anti-Tubulin was detected using a DyLight™ 549 conjugate (red).  Nuclei were counter-stained using DAPI (blue).  The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).
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Synonyms: GST, Glutathione-S-Transferase

GST Antibody Dylight™ 488 Conjugated Properties

Description
Anti-GST (RABBIT) Antibody DyLight™ 488 Conjugated - 600-441-200
Host
Rabbit
Clonality
Polyclonal
Application
FLISA : >1:10,000
IF Microscopy : >1:5,000
Other Dilution: User Optimized
Epitope Tag Type
GST
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
FP Value
4.1 moles DyLight™ 488 per mole of IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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GST Antibody Dylight™ 488 Conjugated Description

Background
GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors.  GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Immunogen
The immunogen is full length GST isolated from Schistosoma japonicum.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Purity/Specifity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].  Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
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Product Label

 

Description
DyLight™ 488
Color
Green
Conjugation Reference
Modified from Thermo Fisher Scientific Inc, Rockford, IL
Molecular Weight
1011
Excitation Wavelength
493
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester