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GFP and Epitopes  >  Sample Size Primary Antibodies
 

RFP Antibody

Chicken Polyclonal
Code

600-901-379S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Western blot of Rockland's Anti-RFP Antibody. 0.1 µg of RFP was loaded on a 4-20% gel and tranferred to nitrocellulose membrane. Anti-RFP Antibody was added at 1.0 µg/mL at RT for 2 hours. IRDye800® anti-Chicken (p/n 603-132-126) was added at 1:20,000 at RT for 45 minutes.
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Shipping:
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Handling:
$20.00
Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

RFP Antibody Properties

Description
Anti-RFP (CHICKEN) Antibody - 600-901-379S
Host
Chicken
Clonality
Polyclonal
Application
ELISA : 1:10,000
Western Blot : 1:2,000 - 1:3,000
Other Dilution: User Optimized
Epitope Tag Type
RFP
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
DsRed
Related Links
Download CofA
Lot#  

RFP Antibody Description

Background
Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including western blotting.
Immunogen
Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
Anti-RFP is designed to detect recombinant RFP. This antibody can be used to detect RFP by immunoblotting. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.
Purity/Specifity
RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by western blot.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Gross LA, Baird GS, Hoffman RC, Baldridge KK, Tsien RY: The structure of the chromophore within DsRed, a red fluorescent protein from coral. Proc. Natl. Acad. Sci. USA 2000, 97: 11990-11995.
Specific Reference
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Product Type
GFP and Epitopes;
Catalog Number
000-001-379

Product Type
GFP and Epitopes;
Applications
ELISA, WB
Catalog Number
600-901-379

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Chicken
Catalog Number
603-103-002

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Chicken
Catalog Number
603-103-126

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry