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Secondary Antibodies  >  Whole IgG Affinity Purified Secondary Antibodies
 

Mouse IgG1 Antibody Biotin Conjugated

Rabbit Polyclonal
Code

610-4640
Size

1 mg
Price

$185.00
Availability

Ships next business day.
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Synonyms: IgG1 Antibody Biotin, Anti IgG1 Antibody Conjugate

Mouse IgG1 Antibody Biotin Conjugated Properties

Description
Anti-MOUSE IgG1 (Gamma 1 chain) (RABBIT) Antibody Biotin Conjugated - 610-4640
Host
Rabbit
Specificity
IgG1
Target Species
Mouse
Clonality
Polyclonal
Application
ELISA : 1:250,000
Western Blot : 1:1,000 - 1:5,000
Immunochemistry: 1:500 - 1:3,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping condition
Ambient
Format
IgG
Label
FP Value
10-20 moles Biotin per mole of IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Lot#  

Mouse IgG1 Antibody Biotin Conjugated Description

Background
Mouse IgG1 secondary antibody conjugated to Biotin is available in a variety of formats. Anti Mouse IgG1 secondary antibody conjugate is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.
Immunogen
Mouse IgG1 heavy chain
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Purity/Specifity
Mouse IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.  Specificity was confirmed by ELISA.  Typically, less than 1% cross-reactivity was observed against other Mouse heavy chain isotypes.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Label

 

Description
Biotin
Color
n/a
Conjugation Reference
Modified from Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
Molecular Weight
455
Excitation Wavelength
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester