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Secondary Antibodies  >  Whole IgG Affinity Purified Secondary Antibodies
 

RABBIT IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Goat Polyclonal
Code

611-1502
Size

1 mg
Price

$149.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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Western Blot of Goat anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated.
Lane 1:  Rabbit IgG.  
Lane 2:  None.
Load:  100 ng per lane.
Primary Antibody:  None.
Secondary antibody:  Alkaline Phosphatase goat secondary antibody at 1:1,000 for 60 min at RT.
Block:  MB-070 for 30 min at RT.
Predicted/Observed size:  55 and 28 kDa, 55 kDa for Rabbit IgG.
Other Band(s):  None.
Dot Blot of Rabbit IgG Antibody Alkaline Phosphatase Conjugated.
Antigen:  Rabbit IgG.  
Load:  Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4ng Lane 5 - 2.5ng.
Primary antibody:  none.
Secondary antibody:  Rabbit IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT.
Block:  MB-070 for 60 min at RT.
Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.
Shipping & Handling Fees Apply.

Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: Rabbit Antibody alkaline phosphatase conjugated, Gt-a-Rabbit alkaline phosphatase conjugated IgG, Rabbit alkaline phosphatase conjugated Antibody in Goat, Rabbit Secondary alkaline phosphatase conjugated Antibody, ALP, Alk Phos.

RABBIT IgG (H&L) Antibody Alkaline Phosphatase Conjugated Properties

Description
Anti-RABBIT IgG (H&L) (GOAT) Antibody Alkaline Phosphatase Conjugated - 611-1502
Host
Goat
Specificity
IgG (H&L)
Target Species
Rabbit
Known Cross Reactivity
Rabbit
Clonality
Polyclonal
Application
ELISA : 1:3,000 - 1:25,000
Western Blot : 1:1,000 - 1:4,000
Immunochemistry: 1:200 - 1:1,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Download CofA
Lot#  

RABBIT IgG (H&L) Antibody Alkaline Phosphatase Conjugated Description

Background
Anti-Rabbit IgG alkaline phosphatase conjugated antibody generated in goat detects specifically rabbit IgG. This secondary alkaline phosphatase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Immunogen
Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in goat.
Immunogen Type
Native Protein
Storage Condition
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Application Note
Anti-Rabbit IgG alkaline phosphatase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunohistochemistry as well as other alkaline phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Purity/Specifity
RABBIT IgG (H&L) Antibody Alkaline Phosphatase conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
Alkaline Phosphatase (Calf Intestine)
Color
Substrate dependent
Conjugation Reference
Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175, 1971.
Molecular Weight
140000
Excitation Wavelength
Conjugation Chemistry
Glutaraldehye coupling