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Anti-GM-CSF (RABBIT) Antibody - 210-401-314
Proteins and Peptides  >  Cytokines and other Growth Factors
 

GM-CSF Human Recombinant Protein

Other - E.coli
Code

009-001-314
Size

20 µg
Price

$225.00
Availability

Ships within 2 weeks.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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SDS-PAGE using Rockland's recombinant human GM-CSF protein shows bands corresponding to GM-CSF  (1µg) in lanes 1 (as unreduced protein) and lane 3 (as reduced protein, see arrowhead).   Molecular weight estimation was made by comparison to prestained MW markers in lane 2 (Invitrogen Mark 12).
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Shipping:
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Handling:
$20.00
Synonyms: Granulocyte Macrophage Colony Stimulating Factor, Granulocyte Macrophage-CSF, Granulocyte-macrophage colony-stimulating factor; GM-CSF cytokine; Colony-stimulating factor; CSF; CSF2; Sargramostim; Molgramostin

GM-CSF Human Recombinant Protein Properties

Description
GM-CSF Human Recombinant Protein - 009-001-314
Host
Other - E.coli
Known Cross Reactivity
Human
Application
Other Dilution: User Optimized
Species of Origin
Human
Physical State
Lyophilized
Shipping condition
Ambient
PSC Type
Cytokine, Interleukin or Growth Factor
PSC Format
Recombinant Protein
Label
Concentration
0.1 mg/mL by UV absorbance at 280 nm
Buffer
0.01 M Sodium Phosphate, pH 7.5
Reconstitution Volume
200 µL to 40 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
GeneName
CSF2
Download CofA
Lot#  

GM-CSF Human Recombinant Protein Description

Background
Granulocyte Macrophage Colony Stimulating Factor is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors , including IL2-beta, IL-3 , IL -6 , IL-7 , EPO and the Prolactin receptors. Recombinant Human Granulocyte Macrophage CSF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular weight of 14.5 kDa.
Storage Condition
Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Application Note
GM-CSF is suitable as a control for polyclonal or monoclonal anti-GM-CSF in immunological assays.
Purity/Specifity
Recombinant protein corresponding to amino acids 18 to 144 of mature human GM-CSF with addition of methionine on the N-terminus. Purity is greater than 98% as determined by reducing and non-reducing SDS-PAGE and by analytical RP-HPLC, each against a known standard.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Grote,K., Schuett,H., Salguero,G., Grothusen,C., Jagielska,J., Drexler,H., Muhlradt,P.F. and Schieffer,B. (2010). Toll-like receptor 2/6 stimulation promotes angiogenesis via GM-CSF as a potential strategy for immune defense and tissue regeneration. Blood 115 (12), 2543-2552.
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-314

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
109-401-314

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
209-401-314

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
mouse
Catalog Number
210-401-314

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry