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Proteins and Peptides  >  Cytokines and other Growth Factors
 

IL-17F Human Recombinant Protein

Other - E.coli
NCI Collaboration
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SDS-PAGE using Rockland's Recombinant Human IL-17F Protein shows bands corresponding to IL-17F  (1µg) in lane 1 (reduced) and lane 3 (unreduced, arrowhead).   Molecular weight estimation was made by comparison to prestained MW markers, lane 2.
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Number:
009-001-B31
Size:
25 µg
Price:
$225.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships within 2 weeks.

IL-17F Human Recombinant Protein Properties

Description
IL-17F Human Recombinant Protein - 009-001-B31
Host
Other - E.coli
Known Cross Reactivity
Human
Application
Other Dilution: User Optimized
Species of Origin
Human
Physical State
Lyophilized
Shipping Condition
Ambient
PSC Type
Cytokine, Interleukin or Growth Factor
PSC Format
Recombinant Protein
Label
Concentration
0.1 mg/mL by UV absorbance at 280 nm
Buffer
0.01 M Sodium Citrate, pH 3.0
Reconstitution Volume
200 µL to 40 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
GeneName
IL17F
Related Links
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IL-17F Human Recombinant Protein Description

Background
Recombinant Human Interleukin-17-F is a non-glycosylated protein consisting of two 134 amino acid polypeptide chains that are cysteine linked homodimeric polypeptide chains with a molecular weight of 30 kDa. Homodimeric interleukin-17F is a member of the IL-17 family of six homodimeric proteins (IL-17A-17F). Activity studies reveal IL-17A to be the most potent, followed by IL-17A/F and IL-17F 100 fold lower than IL-17A.
Synonyms
Interleukin-17F cytokine, IL-17F, Interleukin-24, IL-24, Cytokine ML-1
Storage Condition
Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Application Note
IL-17F is suitable as a control for polyclonal or monoclonal anti-IL-17F in immunological assays.
Purity/Specifity
Purity was determined to be approximately 97% by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Starnes T., Robertson M.J., Sledge G., Kelich S., Nakshatri H., Broxmeyer H.E., Hromas R. J. (2001) IL-17F, a novel cytokine selectively expressed in activated T cells and monocytes, regulates angiogenesis and endothelial cell cytokine production. Immunol. 167:4137-4140 [PubMed: 11591732]
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-B31

Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-B32

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP, Neu
Reacts With
human, primate, dog
Catalog Number
109-401-301

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP
Reacts With
human
Catalog Number
109-401-310

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry