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Proteins and Peptides  >  Cytokines and other Growth Factors
 

IL-35 EBI3 Human Recombinant Protein

Other - E.coli
Code

009-001-B66
Size

20 µg
Price

$225.00
Availability

Ships within 2 weeks.
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SDS-PAGE using Rockland's Recombinant Human EBI-3 Protein shows bands corresponding to EBI-3  (1µg) in lane 1 (unreduced, arrowhead) and lane 3 (reduced).   Molecular weight estimation was made by comparison to prestained MW markers, lane 2.
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Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Epstein-Barr virus induced 3 protein, Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B cytokine, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI-3, EBI3, IL-35, IL35

IL-35 EBI3 Human Recombinant Protein Properties

Description
EBI-3 Human Recombinant Protein - 009-001-B66
Host
Other - E.coli
Known Cross Reactivity
Human
Application
Other Dilution: User Optimized
Species of Origin
Human
Physical State
Lyophilized
Shipping condition
Ambient
PSC Type
Cytokine, Interleukin or Growth Factor
PSC Format
Recombinant Protein
Label
Concentration
0.1 mg/mL by UV absorbance at 280 nm
Buffer
0.01 M Sodium Acetate, pH 4.5
Reconstitution Volume
200 µL to 40 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
0.5% Mannitol
GeneName
IL-27B
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IL-35 EBI3 Human Recombinant Protein Description

Background
Epstein-Barr Virus Induced Gene-3 (EBI-3), is a secreted glycoprotein belonging to the hematopoietin receptor family and related to the p40 subunit of IL-12. It was identified by its induced expression in B-lymphocytes in response to Epstein-Barr virus infection. EBI-3 forms heterodimers with p28 to form IL-27 and with p35 to form IL-35. Both IL-27 and IL-35 have anti-inflammatory and regulatory activity. Recombinant Human EBI is a non-glycosylated polypeptide chain consisting of 209 amino acids with a molecular weight of 23.3 kDa.
Storage Condition
Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Application Note
EBI-3 is suitable as a control for polyclonal or monoclonal anti-EBI-3 in immunological assays.
Purity/Specifity
Recombinant protein corresponds to amino acids 21 to 229 of mature human EBI3. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Kempe,S., Heinz,P., Kokai,E., Devergne,O., Marx,N. and Wirth,T. (2009) Epstein-barr virus-induced gene-3 is expressed in human atheroma plaques . Am. J. Pathol. 175 (1), 440-447. PUBMED 19556516
Specific Reference
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Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry