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Proteins and Peptides > Cytokines and other Growth Factors

IL-7 Human Recombinant Protein

Other - E.coli

Code

009-001-B94

Size

10 µg

Price

$215.00

Availability

Ships next business day.

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Phone: 800.656.7625

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SDS-PAGE shows bands corresponding to IL-7 (1µg) in lane 1 (reduced) and lane 3 (non-reduced, arrowhead). Molecular weight estimation was made by comparison to prestained MW markers, lane 2.

Shipping & Handling Fees Apply.

Shipping:

$40.00 to United States

Handling:

$35.00

Synonyms: Interleukin 7 cytokine, Interleukin-7, IL-7

Datasheet
References
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IL-7 Human Recombinant Protein Properties

Description

IL-7 Human Recombinant Protein - 009-001-B94

Host

Other - E.coli

Known Cross Reactivity

Human

Application

Other Dilution: User Optimized

Species of Origin

Human

Physical State

Lyophilized

Shipping condition

Ambient

PSC Type

Cytokine, Interleukin or Growth Factor

PSC Format

Recombinant Protein

Label

Unconjugated

Concentration

0.1 mg/mL by UV absorbance at 280 nm

Buffer

None

Reconstitution Volume

100 µL to 20 µL

Reconstitution Buffer

Restore with deionized water (or equivalent)

Preservative

None

Stabilizer

None

GeneName

IL7

IL-7 Human Recombinant Protein Description

Background

Interleukin 7 (IL-7), which is constitutively produced particularly by stromal cells from the bone marrow and thymus, plays a crucial role in T cell homeostasis. This cytokine is implicated in thymopoiesis because it sustains thymocyte proliferation and survival. It regulates peripheral naive T cell survival by modulating the expression of the anti-apoptotic molecule Bcl-2, and sustains peripheral T cell expansion in response to antigenic stimulation. Infection by the human immunodeficiency virus (HIV) leads to severe T lymphopenia and general immune dysfunction. Increased IL-7 plasma levels are generally observed in HIV-infected patients. IL-7 may also support bioenergetics that control cell fate decisions in lymphocytes. Recombinant Human IL-7 produced in E. coli is a single, non-glycosylated polypeptide chain containing 153 amino acids and having a molecular mass of 17,412 Daltons.

Storage Condition

Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Application Note

IL-7 is suitable as a control for polyclonal or monoclonal anti-IL-7 in immunological assays

Purity/Specifity

Purity was determined to be greater than 98% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE, against known standard.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Chehtane,M. and Khaled,A.R. (2010) Interleukin-7 mediates glucose utilization in lymphocytes through transcriptional regulation of the hexokinase II gene. Am. J. Physiol., Cell Physiol. 298 (6), C1560-C1571 PUBMED 20200205 Rane,L., Vudattu,N., Bourcier,K., Graniar,E., Hillert,J., Seyfert,V. and Maeurer,M.J. (2010) Alternative splicing of interleukin-7 (IL-7) and interleukin-7 receptor alpha (IL-7Ralpha) in peripheral blood from patients with multiple sclerosis (MS). J. Neuroimmunol. 222 (1-2), 82-86 PUBMED 20226540 Davila,S., Froeling,F.E., Tan,A., Bonnard,C., Boland,G.J., Snippe,H., Hibberd,M.L. and Seielstad,M. (2010) New genetic associations detected in a host response study to hepatitis B vaccine. Genes Immun. 11 (3), 232-238 PUBMED 20237496

Specific Reference

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