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Proteins and Peptides  >  Cytokines and other Growth Factors
 

IL-1ß Mouse Recombinant Protein

Other - E.coli
Code

010-001-319
Size

10 µg
Price

$225.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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SDS-PAGE using Rockland's Recombinant Mouse IL-1ß Protein shows bands corresponding to IL-1ß  (1µg) in lane 4 (unreduced, arrowhead) and lane 2 (reduced).   Molecular weight estimation was made by comparison to prestained MW markers, lanes 1 and 3.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Il1b cytokine, Interleukin-1 beta

IL-1ß Mouse Recombinant Protein Properties

Description
IL-1ß Mouse Recombinant Protein - 010-001-319
Host
Other - E.coli
Known Cross Reactivity
Mouse
Application
Other Dilution: User Optimized
Species of Origin
Mouse
Physical State
Lyophilized
Shipping condition
Ambient
PSC Type
Cytokine, Interleukin or Growth Factor
PSC Format
Recombinant Protein
Label
Concentration
0.1 mg/mL by UV absorbance at 280 nm
Buffer
0.01 M Sodium Phosphate, pH 7.5
Reconstitution Volume
200 µL to 40 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
GeneName
Il1b
Download CofA
Lot#  

IL-1ß Mouse Recombinant Protein Description

Background
Interleukin-1beta (IL-1ß) is a proinflammatory cytokine produced in response to inflammatory agents by a variety of cells, including monocytes, macrophages, and dendritic cells (DCs). IL-1ß and IL-1a are two distinct and independently regulated gene products that comprise IL-1 and signal through the Type 1 IL-1 receptor (IL-1R1). Although IL-1a is cell associated and IL-1ß is secreted, they have nearly identical biological activity in that they induce adhesion molecule expression on epithelial cells, control fever induction, and play a role in arthritis and septic shock. Signaling activated by the IL-1R1 promotes these activities through a MYD88 signaling pathway similar to those associated with Toll receptors. Recombinant mouse IL-1ß is a non-glycosylated single chain 17,394 Da protein containing 152 amino acid residues.
Storage Condition
Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Application Note
Interleukin-1beta (IL-1ß) is suitable as a control for polyclonal or monoclonal anti-Interleukin-1beta (IL-1ß) in immunological assays.
Purity/Specifity
Purity greater than 97% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-301

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP, Neu
Reacts With
human, primate, dog
Catalog Number
109-401-301

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IP, Neu
Reacts With
human, primate, dog
Catalog Number
209-401-301

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, primate, dog
Catalog Number
209-401-B73

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry