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Secondary Antibodies  >  Whole IgG Affinity Purified Secondary Antibodies
 

Monkey IgM (mu chain) Antibody

Goat Polyclonal
Code

617-105-007
Size

1 mg
Price

$215.00
Availability

Ships next business day.
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This table displays additional reactivity among various species of serum and immunoglobulin.  
A (+) indicates antibody reactivity to the corresponding target.
Western Blot of Alkaline Phosphatase Conjugated Goat Anti-Monkey IgM (mu chain) Secondary Antibody.
Lane 1:  Monkey IgM.
Lane 2:  None.
Load:  100 ng per lane.
Primary antibody:  None.
Secondary antibody:  Alkaline Phosphatase goat secondary antibody at 1:1,000 for 60 min at RT.
Blocking:  MB-070 for 30 min at RT.
Predicted/Observed size:  72 kDa, 72 kDa for Monkey IgM.
Other band(s):  Monkey IgM splice variants and isoforms.
Shipping & Handling Fees Apply.

Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: ALP, Alk Phos, Goat Anti-Monkey IgM Antibody, Gt-a-Monkey IgM, Monkey IgM Secondary Antibody

Monkey IgM (mu chain) Antibody Properties

Description
Anti-MONKEY IgM (mu chain) (GOAT) Antibody Alkaline Phosphatase Conjugated - 617-105-007
Host
Goat
Specificity
IgM µ chain
Target Species
Monkey
Clonality
Polyclonal
Application
ELISA : 1:2,000 - 1:10,000
Western Blot : 1:500 - 1:3,000
Immunochemistry: 1:200 - 1:1,000
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Format
IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Lot#  

Monkey IgM (mu chain) Antibody Description

Background
Anti-Monkey IgM (mu chain) Antibody generated in goat detects specifically mouse IgM whole molecule. Anti-Monkey IgM (mu chain) Antibody is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Monkey IgM (mu chain) Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
Immunogen
Monkey IgM mu heavy chain
Storage Condition
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Application Note
Anti-Monkey IgM (mu chain) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.Specific conditions for ractivity should be optimized by the end user.
Purity/Specifity
Anti-Monkey IgM (mu chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Monkey IgM and Monkey Serum.   Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Janeway, Jr., Travers, Walport, and Shlomchik. "The Immune System in Health and Disease." Immunobiology, 5th Edition: Garland Science: 2001.
Specific Reference
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Product Type
Proteins and Peptides;
Catalog Number
017-0202

Product Type
Proteins and Peptides;
Catalog Number
017-0302

Product Type
Proteins and Peptides;
Catalog Number
017-0502

Product Type
Proteins and Peptides;
Catalog Number
017-0602

Product Label

 

Description
Alkaline Phosphatase (Calf Intestine)
Color
Substrate dependent
Conjugation Reference
Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175, 1971.
Molecular Weight
140000
Excitation Wavelength
Conjugation Chemistry
Glutaraldehye coupling