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Progesterone Receptor Antibody

Rabbit Monoclonal SP2 IgG
NCI Collaboration
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Progesterone Receptor (PR) monoclonal antibody stains Human Breast Carcinoma.
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Number:
900-C01-B36
Size:
100 µL
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Progesterone Receptor Antibody Properties

Description
Anti-PROGESTERONE RECEPTOR (RABBIT) Monoclonal Antibody - 900-C01-B36
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Monoclonal SP2 IgG
Application
ELISA : 1:10,000
Western Blot : 1:200-1:500
Immunohistochemistry: 1:400
Physical State
Liquid (sterile filtered)
Shipping Condition
Wet Ice
Format
CCS
Label
Concentration
N/A
Buffer
See application note.
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
GeneName
PGR
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Progesterone Receptor Antibody Description

Background
The PGR gene encodes a member of the steroid receptor superfamily referred to as progesterone receptor (also known as PgR, PR and nuclear receptor subfamily 3 group C member 3). Progesterone receptor mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform A only, and mediate their own response genes and physiologic effects with little overlap. Ideal for Cancer, Chromatin & Nuclear Signaling and Signal Transduction research.
Synonyms
PgR PR nuclear receptor subfamily 3 group C member 3
Immunogen
Anti-Progesterone Receptor monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 412-526 of human Progesterone Receptor protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.
Immunogen Type
Recombinant Protein
Storage Condition
Store at 2-8°C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date.
Application Note
This monoclonal antibody contains TBS/1% BSA and is suitable for ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 116 kDa in size corresponding to PgR isoform B (the ‘canonical’ sequence) protein by western blotting in the appropriate cell lysate or extract. Progesterone Receptor is found in human Breast ductal carcinoma and is a nuclear protein. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min.
Purity/Specifity
This product is clarified tissue culture supernate. The antibody is specific for human Progesterone Receptor protein. Cross-reactivity with Progesterone Receptor protein from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Kastner P., Krust A., Turcotte B., Stropp U., Tora L., Gronemeyer H., Chambon P. (1990) Two distinct estrogen-regulated promoters generate transcripts encoding the two functionally different human progesterone receptor forms A and B. EMBO J. 9:1603-1614. Misrahi M., Atger M., D'Auriol L., Loosfelt H., Meriel C., Fridlansky F., Guiochon-Mantel A., Galibert F., Milgrom E. (1987) Complete amino acid sequence of the human progesterone receptor deduced from cloned cDNA. Biochem. Biophys. Res. Commun. 143:740-748. Chen C., Opazo J.C., Erez O., Uddin M., Santolaya-Forgas J., Goodman M., Grossman L.I., Romero R., Wildman D.E. (2008) The human progesterone receptor shows evidence of adaptive evolution associated with its ability to act as a transcription factor. Mol. Phylogenet. Evol. 47:637-649. Knotts T.A., Orkiszewski R.S., Cook R.G., Edwards D.P., Weigel N.L. (2001) Identification of a phosphorylation site in the hinge region of the human progesterone receptor and additional amino-terminal phosphorylation sites. J. Biol. Chem. 276:8475-8483.
Specific Reference
Cano G, Milanezi F, Leitão D, Ricardo S, Brito MJ, Schmitt FC. (2003) Estimation of Hormone Receptor Status in Fine-Needle Aspirates and Paraffin-embedded Sections from Breast Cancer Using theNovel Rabbit Monoclonal antibodies SP1 and SP2. Diaqn Cytopathol 4:207-11. Huang Z, Zhu W, Szekeres G, Xia H. (2005) Development of new rabbit monoclonal antibody to estrogen receptor (clone SP1): Immunohistochemical assessment on formalin-fixed, paraffin-embedded tissue sections. Appl Immunohistochem Mol Morphol. 1:91-5.
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
900-C01-B35

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry