14-12_peptide top banner
 
Avidin, Biotin, Streptavidin  >  Avidin-Biotin Reagents
 

Avidin Glucose Oxidase Conjugated

NCI Collaboration
Order Offline
Number:
A003-07
Size:
2 mg
Price:
$115.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

Avidin Glucose Oxidase Conjugated Properties

Description
AVIDIN GLUCOSE OXIDASE Conjugated - A003-07
Application
ELISA : 1:8,000 - 1:32,000
Western Blot : 1:500 - 1:2,500
Immunohistochemistry: 1:200 - 1:1,000
Other Dilution: User Optimized
Reagent Type
Avidin
Physical State
Lyophilized
Shipping Condition
Ambient
Concentration
2.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Download CofA:
Lot#  
Print

Avidin Glucose Oxidase Conjugated Description

Background
Avidin is a biotin-binding protein found in the oviducts of egg-laying animals (birds, reptiles, and frogs) that gets deposited into the whites of their eggs. Avidin is a tetramer and can bind up to four biotin molecules (Vitamin B7) with one of the greatest known non-covalent interactions. Avidity for biotin is destroyed with heat. Glucose oxidase is an enzyme that drives the oxidation of glucose into hydrogen peroxide and D-glucono-d-lactone. This activity is utilized in biotechnology through colormetric detection assays that are sensitive to the amount of hydrogen peroxide produced. Avidin Glucose Oxidase Conjugated is ideal for investigators in Immunology, Cancer, Neuroscience, and Cell Biology.
Synonyms
AvD, Avidin Biotin Complex, GOD, GOx
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
Avidin Glucose Oxidase Conjugated is a useful detection reagent for primary antibodies conjugated to biotin. Avidin Glucose Oxidase Conjugated can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).
Purity/Specifity
Avidin Glucose Oxidase was prepared from chromatographically pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin and anti-Glucose Oxidase.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Lambros and Lee. (1988). "Use of avidin-biotin-glucose oxidase complex to detect antimalarial antibody in serum by light microscopy." Am J Trop Med Hyg. 1988. Aug. 39 (2) : 145-9. Hsu, Raine, and Fanger. (1981). Use of Avidin-Biotin-Peroxidase Complex (ABC) in Immunoperoxidase Techniques: A Comparison Between ABC and Unlabeled Antibody (PAP) Procedures. Journal of Histochem Cytochem. Apr. 29, (4):577-80.
Specific Reference
Log in to submit your product review.

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Custom Products;
Applications
ELISA, WB, IP
Catalog Number
CUST09

Product Label

 

Description
Glucose Oxidase (Aspergillus niger)
Color
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
160000
Excitation Wavelength
Conjugation Chemistry
Periodate