House TrueBlot GOC
 
You may also want to consider these related products.
Anti-GLUCOSE OXIDASE (GOAT) Antibody - 100-1137
Anti-GLUCOSE OXIDASE (GOAT) Antibody - 100-1137
AVIDIN - A003-01
AVIDIN - A003-01
AVIDIN GLUCOSE OXIDASE Conjugated - A003-07
AVIDIN GLUCOSE OXIDASE Conjugated - A003-07
BIOTIN CONJUGATION (up to 20 mg) - CUST09
BIOTIN CONJUGATION (up to 20 mg) - CUST09
Avidin, Biotin, Streptavidin  >  Avidin-Biotin Reagents
 

Biotin Glucose Oxidase Conjugated

Code

B000-07
Size

5 mg
Price

$95.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Dot Blot of BIOTIN GLUCOSE OXIDASE Conjugated.  
Lane 1: Biotin Conjugated Glucose Oxidase.
Lane 2: Glucose Oxidase.  
Load:  3-fold serial dilution starting at 200 ng.
Primary Antibody:  None.
Secondary antibody:  HRP Streptavidin at 1:40,000 for 30 min at RT.
Block:  1% BSA-TTBS 30 min at RT.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Vitamin H, Coenzyme R, Vitamin B7, GOx, GOD

Biotin Glucose Oxidase Conjugated Properties

Description
BIOTIN GLUCOSE OXIDASE Conjugated - B000-07
Application
ELISA : 1:8,000 - 1:32,000
Western Blot : 1:500 - 1:2,500
Immunochemistry: 1:200 - 1:1,000
Other Dilution: User Optimized
Reagent Type
Biotin
Physical State
Lyophilized
Shipping condition
Ambient
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
5.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Download CofA
Lot#  

Biotin Glucose Oxidase Conjugated Description

Background
Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, Biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin; an attraction that is the strongest and most stable non-covalent interaction known. Glucose oxidase is an enzyme that drives the oxidation of glucose into hydrogen peroxide and D-glucono-d-lactone. This activity is utilized in biotechnology through colormetric detection assays that are sensitive to the amount of hydrogen peroxide produced. Biotin Glucose Oxidase Conjugated is ideal for investigators involved in Cancer, Cell Signaling, Neuroscience, and Cell Biology research.
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Biotin Glucose Oxidase Conjugated is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Biotin Glucose Oxidase Conjugated can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.
Purity/Specifity
Biotin Glucose Oxidase Conjugated was prepared from chromatographically purified biotin.  Biotin Glucose Oxidase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Glucose Oxidase and anti-Biotin.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Wilson, R. and A. P. F. Turner. (1992). Glucose Oxidase: An Ideal Enzyme. Biosensors and Bioelectronics. Vol. (7): 165-185. de Boer, Rodriguez, and Bonte et al. (2003). Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice. Proc Natl Acad Sci U S A. 2003 June 24; 100(13): 7480–7485.
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Aspergillus niger
Catalog Number
100-1137

Product Type
Avidin, Biotin, Streptavidin;
Applications
ELISA, WB, GS, ChIP, Other
Catalog Number
A003-01

Product Type
Avidin, Biotin, Streptavidin;
Applications
ELISA, WB, IHC
Catalog Number
A003-07

Product Type
Custom Products;
Applications
ELISA, WB, IP
Catalog Number
CUST09

Product Label

 

Description
Biotin
Color
n/a
Conjugation Reference
Modified from Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
Molecular Weight
455
Excitation Wavelength
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester