Biotin Glucose Oxidase Conjugated Dot Blot

Microsensor Biochemistry using Biotin Glucose Oxidase

Datasheet

Biotin Glucose Oxidase Conjugated

Name: Biotin Glucose Oxidase Conjugated
Brand: Rockland Immunochemicals
Price: 132.0 USD
Availability: InStock

2 References

Item No. B000-07

Size: 5 mg

Applications: ELISA, Dot Blot, Microarray, Other

$132.00 /Per Item

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Background

Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, Biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin; an attraction that is the strongest and most stable non-covalent interaction known. Glucose oxidase is an enzyme that drives the oxidation of glucose into hydrogen peroxide and D-glucono-δ-lactone. This activity is utilized in biotechnology through colormetric detection assays that are sensitive to the amount of hydrogen peroxide produced. Biotin Glucose Oxidase Conjugated is ideal for investigators involved in Cancer, Cell Signaling, Neuroscience, and Cell Biology research.

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Specifications for Biotin Glucose Oxidase Conjugated

Product Details

Description: Biotin Glucose Oxidase Conjugated - B000-07

Synonyms: Biotin Glucose Oxidase Conjugation, Glucose Oxidase Conjugated Biotin, Biotin conjugated Glucose Oxidase

Conjugate: Biotin

Target Details

Purity/Specificity: Biotin Glucose Oxidase Conjugated was prepared from chromatographically purified biotin. Biotin Glucose Oxidase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Glucose Oxidase and anti-Biotin.

Application Details

Tested Applications: Dot Blot, ELISA

Suggested Applications: Microarray, Other - View References

Application Note: Biotin Glucose Oxidase Conjugated has been tested by ELISA and dot blot and can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.

Formulation

Physical State: Lyophilized

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Reconstitution Volume: 5.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Biotin Glucose Oxidase Conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (2)

Kemp E et al. (2022). Influence of enzyme immobilization and skin-sensor interface on non-invasive glucose determination from interstitial fluid obtained by magnetohydrodynamic extraction. Biosens Bioelectron.

Applications

Ab Immobilization

PubMed

Amarie D et al. (2012). Label-free microcavity biosensors: steps towards personalized medicine. Sensors (Basel, Switzerland)

Applications

Microarray

PubMed

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

General Immunoblotting Protocol

Sandwich ELISA Protocol for Collagen

Suspension Cultured Cell Lysis Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.