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Supporting Reagents  >  Blocking Solutions and BSA
 

Bovine Serum Albumin - Fraction V

Code

BSA-1000
Size

1 kg
Price

$925.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
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This product is suitable for use in protease sensitive assays such as RIA, EIA and nucleic acid hybridization, use as a stabilizing agent for proteins and enzymes including dilute solutions of antibody, use as a blocking agent to reduce non-specific binding.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Bovine Serum Albumin, BSA, BSA Blocker, BSA Blocking, FACS Buffer

Bovine Serum Albumin - Fraction V Properties

Description
BOVINE SERUM ALBUMIN - Fraction V (Immunoglobulin and Protease Free) - BSA-1000
Application
ELISA : Yes
FLISA : Yes
IF Microscopy : Yes
FlowCytometry : Yes
Western Blot : Yes
Immunochemistry: Yes
ImmunoPrecipitation: Yes
Other Dilution: User Optimized
Blocker Type
BSA
Substrate/Enzyme Type
Blocking Reagent
Product Type
Blocking Reagent
Physical State
Lyophilized
Shipping condition
Ambient
Label
Buffer
None
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
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Lot#  

Bovine Serum Albumin - Fraction V Description

Background
Bovine Serum Albumin (BSA) is used for various biochemical applications including ELISA (Enzyme-Linked Immunosorbent Assay), high content screenining assays, western blotting, and immunohistochemistry. BSA as a blocking reagent is particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Bovine Serum Albumin can also be used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA.
Storage Condition
Store BSA at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Bovine Serum Albumin - Fraction V is suitable for use in protease sensitive assays such as RIA, EIA and nucleic acid hybridization, use as a stabilizing agent for proteins and enzymes including dilute solutions of antibody, use as a blocking agent to reduce non-specific binding. BSA is also ideal for the production of FACS buffer 0.5% BSA w/ 0.05% Azide in PBS.
Purity/Specifity
Purity (%): 100% by Agarose Zone Electrophoresis Moisture (%): <0.9% pH: 7.0 Protein (%): 100.5% by nitrogen analysis. Sodium Chloride (mg/g): <5.0 by titration Protease: <0.005 units/mg by Enzymatic Assay. Immunoglobulin: None Detected by Radial Immunodiffusion. Heavy Metals: <0.49 ppm
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry