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Biotin Conjugation

NCI Collaboration
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Western Blot of Rabbit anti-Human IgG (H&L) Antibody Biotin Conjugated

Lane 1:  Human IgG

Load:  50 ng per lane

Primary antibody: Human IgG (H&L) Antibody Biotin Conjugated at 1:1000 for 60 min RT

Secondary antibody:  HRP Conjugated Streptavidin at 1:40,000 for 30 min at RT

Block:  MB-070 for 30 min at RT
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Number:
CUST09
Size:
1 Each
Price:
Price On Request
Availability:
Ships next business day.

Biotin Conjugation Properties

Description
BIOTIN CONJUGATION (up to 20 mg) - CUST09
Application
ELISA : User Optimized
Western Blot : User Optimized
ImmunoPrecipitation: User Optimized
Other Dilution: User Optimized
Physical State
Liquid
Shipping Condition
Wet Ice
Label
Concentration
1.8 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Biotin Conjugation Description

Background
Supply up to 20 mg of antibody and we will conjugate to biotin using a long arm spacer biotin derivative, and purify resulting in a 10-15:1 ratio. Biotin conjugated antibodies are routinely used in Western Blotting, ELISA and IHC protocols.
Synonyms
BAC, biotin-epsilon-aminocaproic acid
Purity/Specifity
This product was prepared from provided antibody by conjugation to biotin and subsequent purification.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Label

 

Description
Biotin
Color
n/a
Conjugation Reference
Modified from Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
Molecular Weight
455
Excitation Wavelength
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester