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Custom Products  >  Antibody Conjugation
 

Peroxidase Conjugation

Code

CUST10
Size

1 Each
Price

Price On Request
Availability

Ships next business day.
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Western Blot of Rabbit anti-GOAT IgG (H&L) Antibody Peroxidase Conjugated

Lane 1:  Goat IgG

Load:  100 ng per lane

Secondary antibody:  GOAT IgG (H&L) Antibody Peroxidase Conjugated at 1:1,000 for 60 min at RT

Block:  MB-070 for 30 min at RT
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Synonyms: HRP, Horseradish Peroxidase, Horse Radish Peroxidase

Peroxidase Conjugation Properties

Description
PEROXIDASE CONJUGATION (up to 20 mg) - CUST10
Application
ELISA : User Optimized
Western Blot : User Optimized
ImmunoPrecipitation: User Optimized
Other Dilution: User Optimized
Physical State
Liquid
Shipping condition
Wet Ice
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Lot#  

Peroxidase Conjugation Description

Background
Custom HRP conjugation of up to 20 mg of affinity, Protein A or Protein G purified antibody. The antibody is conjugated to peroxidase through the CHO group and typically yield a ratio of 2:1. Horse Radish Peroxidase conjugated antibodies are routinely used in Western Blotting, ELISA and IHC protocols.
Purity/Specifity
This product was prepared from provided antibody by conjugation to peroxidase and subsequent purification.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Label

 

Description
Peroxidase (Horseradish)
Color
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
40000
Excitation Wavelength
Conjugation Chemistry
Periodate