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Protein G Purification



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Antibody-binding protein specificity for Protein A, Protein G, and Protein A/G which demonstrate binding specificity to IgG immunoglobulin through an interaction in the Fc region of the heavy chain domain. Protein A and G each show some preferences to certain types of antibody subclasses, and proper reagent selection may be required, Protein A/G offers the widest range of antibody subclass binding. Another immunoglobulin binding protein is Protein L which binds to the kappa light chain of the F(ab’)2 region.
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Synonyms: Protein G chromatography, IgG purification, IgG fractionation, antibody purification, purification

Protein G Purification Properties

PROTEIN G PURIFICATION (up to 100 ml serum or 100 ml of ascites or 500 ml of processed and concentrated tissue culture supernate) - CUST19
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Wet Ice
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Protein G Purification Description

Protein G is a protein that has the property of binging to immunoglobulins. It is a 65-kDa cell surface protein that is commonly used for purifying antibodies through binding to the Fab and Fc regions. Protein G was originally isolated from Streptococcal bacteria. It is similar in properties to Protein A but has unique IgG binding specificities. Native protein G also binds albumin, however Rockland uses recombinant forms of Protein G that only bind to immunoglobulins.
Application Note
Protein G purification is a process for isolating the IgG type of immunoglobulin protein from a complex mixture. The starting material is usually but not limited to polyclonal antisera, tissue culture supernatant, or ascites fluid. Typically protein G purification is performed when IgG enrichment is desired, and is often used to purify IgG prior to conjugation to a reporter molecule. Protein G may offer improved purifcation compared to Protein A for certain immunoglobulin classes and for certain host organisms. The IgG is bound to an immobilized Protein G matrix, non-IgG impurities are wased away, lastly the IgG fraction is eluted and recovered. Rockland's Protein G purification process is ideal to recover a highly active IgG fraction from complex starting material.
Protein G chromatography is performed on up to 100 mL of antiserum, ascites or concentrated tissue culture supernatant to purify immunoglobulins known to bind (including certain mouse subclasses) Protein G.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Streptococcal Protein G Gene Structure and Binding Properties, (1991) J Biol. Chem. V266 pp 399-405 Ulf Sjobring, Lars Bjorck, and William Kastern
Specific Reference
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Product Type
Primary Antibodies;
Reacts With
human, mouse, rat, monkey
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Product Type
Secondary Antibodies;
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Product Type
Supporting Reagents;
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Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry