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Protein A Purification

NCI Collaboration
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Antibody-binding protein specificity for Protein A, Protein G, and Protein A/G which demonstrate binding specificity to IgG immunoglobulin through an interaction in the Fc region of the heavy chain domain. Protein A and G each show some preferences to certain types of antibody subclasses, and proper reagent selection may be required, Protein A/G offers the widest range of antibody subclass binding. Another immunoglobulin binding protein is Protein L which binds to the kappa light chain of the F(ab’)2 region.
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Number:
CUST22
Size:
1 Each
Price:
Price On Request
Availability:
Ships within 2 weeks.

Protein A Purification Properties

Description
PROTEIN A PURIFICATION (up to 100 ml serum or 100 ml of ascites or 500 ml of processed and concentrated tissue culture supernate) - CUST22
Application
ELISA : User Optimized
ChIP : User Optimized
IF Microscopy : User Optimized
Neutralization : User Optimized
Flow Cytometry : User Optimized
Western Blot : User Optimized
Immunohistochemistry: User Optimized
ImmunoPrecipitation: User Optimized
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Wet Ice
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Protein A Purification Description

Background
Protein A is 56 kDa protein molecule found in the cell wall of the bacterium Staphylococcus aureus. Protein A binds to immunoglobulins through interaction with the IgG heavy chain within the Fc region. Protein A does not bind to all IgG's equivalently and binding varies among species. Even within a species, Protein A interacts with some subclasses of IgG and not others. Protein A is well suited for purification of most rabbit, mouse, rat, and certain human IgG class of immunoglobulin. Rockland offers Protein G or recombinant Protein A/G when recommended by Rockland for optimal purifcation.
Synonyms
purification, IgG fraction, antibody purification, IgG fractionation
Application Note
Protein A purification is a process for isolating the IgG type of immunoglobulin protein from a complex mixture. The starting material is usually but not limited to polyclonal antisera, tissue culture supernatant, or ascites fluid. Typically protein A purification is performed when IgG enrichment is desired, and is often used to purify IgG prior to conjugation to a reporter molecule. The IgG is bound to an immobilized Protein A matrix, non-IgG impurities are wased away, lastly the IgG fraction is eluted and recovered. Rockland's Protein A purification process is ideal to recover a highly active IgG fraction from complex starting material.
Purity/Specifity
Chromatography is performed on up to 100 mL of antiserum, ascites or concentrated tissue culture supernatant to purify immunoglobulins known to bind (including certain mouse subclasses) Protein A.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Differences in anti-protein A activity among IgG subgroups. J Immunol 103(4):828-33; Kronvall, G. and Williams, R.C. (1969) Protein A: Nature's universal anti-body. Trends in Biochem Sci 7: 74-76. Surolia, A., et al. (1982).
Specific Reference
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Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry