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Datasheet Protocol

RFP Chemiluminescent Western Blot Kit

1 References
KCA379
1 Each
WB
RFP, mBanana, mCherry, mOrange, mPlum, mStrawberry, rRFP, tdTomato
$419.00 /Per Item
Availability: Call for availability
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Red Fluorescent Protein (RFP) Western Blotting kit allows for the detection of RFP-tagged recombinant proteins present in cell lysates provided by the user. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-RFP antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.

Product Details

RFP Western Blot Kit: for RFP Chemiluminescent Western Blotting - KCA379
Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit, RFP, rRFP, mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange, mStrawberry
Polyclonal
Chemiluminescent Western Blot Kit

Target Details

dsRed  - View All dsRed Products
RFP, mBanana, mCherry, mOrange, mPlum, mStrawberry, rRFP, tdTomato
Rockland Immunochemicals’ Chemiluminescent Western Blot Kit for RFP combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol chemiluminescent substrate for the detection of recombinant proteins containing wild type Red Fluorescent Protein (DsRed or RFP) and its variants such as mBanana, mCherry, DsRed2, E2-Crimson, mOrange, mOrange2, mPlum, mRaspberry, mStrawberry and tdTomato. The Chemiluminescent Western Blot Kit design includes straightforward procedures and color-coding to allow for ease of use. This kit contains sufficient substrate for up to 30 mini blots at 7.5 x 8 cm2 (1,800 cm2) and is stable for at least 1 year when stored as indicated.

Application Details

WB
Use Rockland Immunochemicals’ Anti-RFP Chemiluminescent Kit for Western Blotting for detection of RFP-tagged recombinant proteins by western blot. This kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.

Formulation

Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!

Shipping & Handling

Wet Ice
See kit insert for complete instructions.
See kit insert for complete instructions.
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Applications
WB, IB, PCA

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