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Kits  >  Immunohistochemistry IHC Kits
 

Immunohistochemistry Kit: IHC Kit for Rabbit Primary Antibody MaxTag™ Histo

Code

KHA003
Size

1 Kit
Price

$315.00
Availability

Ships within 2 weeks.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
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IHC kit with DAB substrate can generate publication quality results for novices and experts alike.  Expect reliable and reproducible signal with low background staining when using the MaxTag™ Histo IHC Kit.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: IHC Kit, Peroxidase Kit, Immunohistochemistry Kit

Immunohistochemistry Kit: IHC Kit for Rabbit Primary Antibody MaxTag™ Histo Properties

Description
Immunohistochemistry Kit: IHC Kit for Rabbit Primary Antibody MaxTag™ Histo - KHA003
Known Cross Reactivity
Anti-Rabbit IgG
Application
Immunohistochemistry: 1:1,000
Other Dilution: User Optimized
Product Type
Kit
Kit Type
Immunohistochemistry Kit
Physical State
n/a
Shipping condition
Ambient
Label
Concentration
Sufficient to run approximately 100-200 immunostains on standard microscope slides.
Preservative
Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!
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Lot#  

Immunohistochemistry Kit: IHC Kit for Rabbit Primary Antibody MaxTag™ Histo Description

Background
This MaxTag Histo Immunohistochemistry Kit allows for the detection of primary rabbit polyclonal or monoclonal antibody provided by the user. IHC kit relies upon the high specificity of avidin-biotin binding for visualization of an antigen. The antigen (usually within immobilized cells or tissue) is fixed and adhered to a glass microscope slide. A primary antibody reacts with the immobilized antigen to form an antigen-antibody complex. A second biotinylated antibody derived from goat and specific for the primary antibody reacts with the complex. Streptavidin conjugated to the enzyme peroxidase reacts with this complex localizing the enzyme at the site of the antigen. Finally, substrate is added causing a reaction with the peroxidase as a colored precipitate to form on the slide at the location of the antigen. The slide is viewed through a standard light microscope and, if desired, photographed for a permanent record.
Storage Condition
See kit insert for complete instructions.
Application Note
Rockland Immunochemicals' MaxTag Histo Immunohistochemistry Kit is intended to provide a simple, reliable and convenient detection system for immunohistochemical staining. As such, the kit can be used for the identification of specific constituents in tissue sections or immobilized cells. In order to detect the reaction site, the antibody complex is labeled with an enzyme (peroxidase) that can be reacted with a suitable substrate (DAB) to give a colored product. This kit also provides for the counter staining of nuclei to reveal tissue architecture. The stain and substrate color fade slowly, if at all, allowing the slides to be stored for future use. The contents of this kit are formulated for maximum ease of use and are color coded to eliminate confusion. A sample protocol and recommended conditions for use are provided. Formaldehyde fixation is suggested as the routine initial method of choice for tissue and cell fixation. The provided protocol is a guideline. Any procedure can be altered according to specific experimental requirements. The use of both highly purified and adsorbed antibody conjugates and matched reagents reduces any lot-to-lot variation. This kit can process 100-200 immunostains on standard microscope slides and is stable for at least 1 year when stored at +4°C.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Kits;
Applications
IHC
Reacts With
Anti-Human IgG
Catalog Number
KHA001

Product Type
Kits;
Applications
IHC
Reacts With
Anti-Mouse IgG
Catalog Number
KHA002

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry