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Molecular Biology Reagents  >  Water and Molecular Biology Solutions
 

10X TTBS pH 7.5

Code

MB-013
Size

1 L
Price

$70.00
Availability

Ships next business day.
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E-mail: orders@rockland-inc.com
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Suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers. This buffer consists of 1.0 M Tris HCl, 1.5 M Sodium Chloride and 0.1% (w/v) Tween-20 at a pH of 7.5.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$35.00
Synonyms: TTBS, TBST, Tween Tris Buffered Saline

10X TTBS pH 7.5 Properties

Description
10X TTBS pH 7.5 (1.0 M Tris HCl 1.5 M Sodium Chloride 0.1% (v/v) Tween-20) - MB-013
Product Type
Buffer
Product Type
Solution
Physical State
Liquid (sterile filtered)
Shipping condition
Ambient
Label
Buffer
See application note.
Preservative
None
Stabilizer
None
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Lot#  

10X TTBS pH 7.5 Description

Background
Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.
Storage Condition
Store container at room temperature (18° to 26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility of some salts.
Application Note
Suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers. This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 1.0 M Tris HCl, 1.5 M Sodium Chloride and 0.1% (v/v) Tween-20 at a pH of 7.5.  Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.
Purity/Specifity
10X TTBS solution was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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AMCA Conjugates
Antigen Retrieval Methods
Azide Removal by Dialysis
Beta Galactosidase Conjugates
Biotin Binding Assay (HABA)
Biotin Conjugates
Cell Lysates Protocol
CyDye™ Conjugates
DyLight™ Antibody Conjugate Properties
DyLight™ Antibody Spectra
ELISA
ELISA to Quantify Collagen
Extended Conjugate Information Sheet
Fab Fragment Monovalent Antibodies
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Gel Shift Assay
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Histone Immunoblotting
Immunoblotting
Immunocytochemistry
Immunofluorescence Microscopy
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Immunofluorescence Microscopy - Fluorescent Labels
Immunofluorescence Microscopy - IRDye®
Immunofluorescence Microscopy - long
Immunofluorescence Microscopy - Microscope Types
Immunofluorescence Microscopy - Other Conjugates
Immunofluorescence Microscopy Protocol
Immunohistochemistry
Immunoprecipitation
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Peroxidase & Alkaline Phosphatase
Phosphorylated State Proteins
Phosphorylated State Proteins - AKTs
Phosphorylated State Proteins - Long
Phosphorylated State Proteins - Pathways
Phosphorylated State Proteins - Phospho Specific
Phosphorylated State Proteins - Tailored Phosphorylated Target
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Rhodamine & Texas Red™
SDS-PAGE
Staining Paraffin Sections by PAP Procedure
USDA & Material Safety Notice
Western Blot - Buffers
Western Blot - Long
Western Blot - Preassembled Kits
Western Blot - Primary Antibody Reagents
Western Blot - Secondary Antibody Reagents
Western Blot - Substrates
Western Blot - Types of Blot Formats
Western Blot (Protein Immunoblot)
Product Type
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Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
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Applications
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Catalog Number
B304

Product Type
Molecular Biology Reagents;
Catalog Number
MB-008

Product Type
Molecular Biology Reagents;
Catalog Number
MB-009-1000

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry