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Molecular Biology Reagents  >  Water and Molecular Biology Solutions
 

10X SDS-PAGE Running Gel Buffer

Code

MB-017
Size

1 L
Price

$75.00
Availability

Ships next business day.
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SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative charge to linearized proteins.
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Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: Sodium dodecyl sulfate polyacrylamide running buffer, Protein Gel Running Buffer, polyacrylamide gel running buffer.

10X SDS-PAGE Running Gel Buffer Properties

Description
10X SDS-PAGE Running Gel Buffer (0.25 M Tris, 1.92 M Glycine, 1.0% SDS pH 8.3) - MB-017
Application
Other Dilution: User Optimized
Product Type
Buffer
Product Type
Solution
Physical State
Liquid (sterile filtered)
Shipping condition
Ambient
Label
Buffer
See application note.
Preservative
None
Stabilizer
None
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Lot#  

10X SDS-PAGE Running Gel Buffer Description

Background
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative charge to linearized proteins.
Storage Condition
Store container at room temperature (18° to 26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility of some salts.
Application Note
SDS-PAGE running buffer is suitable for laboratory involved in protein biochemistry. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers. This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water (or equivalent) to its final working concentration. No pH adjustment is required. 10X SDS-PAGE Running Buffer consists of 0.25 M Tris HCl, 1.92 M Glycine and 1% (w/v) Sodium Dodecyl Sulfate (SDS); pH 8.3.  Meticulously prepared using ultra-pure reagents dissolved in highly polished pharmaceutical grade deionized water.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Molecular Biology Reagents;
Catalog Number
MB-001

Product Type
Molecular Biology Reagents;
Catalog Number
MB-006

Product Type
Molecular Biology Reagents;
Catalog Number
MB-008

Product Type
Molecular Biology Reagents;
Catalog Number
MB-009-1000

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry