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Molecular Biology Reagents  >  Water and Molecular Biology Solutions
 

HRP Conjugate Stabilizer

Code

MB-060-1000
Size

1 L
Price

$179.00
Availability

Ships within 2 weeks.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Rockland Immunochemicals produces a wide variety of buffers and substrates for use in ELISAs. Antigen was diluted in ELISA Microwell Coating Stabalizer (p/n MB-063-0100) added to the microwell plate and incubated overnight at 4°C. The plate was then blocked with ELISA Microwell Blocking Buffer with Stabilizer (p/n MB-064-1000) for 2 hours. The primary antibody was diluted in PBS Fish Gel Concentrate (1:10)(p/n MB-066-0100), added to the plate, and allowed to incubate 1 hour at room temperature. HRP conjugated secondary antibody was diluted in HRP Conjugate Stabilizer (p/n MB-060-0100), added to the plate, and allowed to incubate for 30 minutes at room temperature. TMB ELISA Peroxidase Substrate (p/n TMBE-1000) was added to the plate and allowed to incubate for 30 minutes at room temperature. The reaction was then stopped with 1M HCl and read at 450nm.
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Synonyms:

HRP Conjugate Stabilizer Properties

Description
HRP Conjugate Stabilizer (Azide and Mercury Free) - MB-060-1000
Product Type
Buffer
Product Type
Solution
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Label
Concentration
N/A
Buffer
See application note.
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Lot#  

HRP Conjugate Stabilizer Description

Background
HRP Conjugate Stabilizer is to be used with Horseradish Peroxidase (HRP) conjugated primary or secondary antibodies or other proteins or compounds in solution.  The components of the stabilizer (proprietary) are unique, and stabilize both the antibody and enzyme portions of the conjugate. HRP Conjugate Stabilizer prevents the loss of catalytic activity and maintains the active conformation of the antibody portion of the conjugate.
Storage Condition
Store container at 4° C before opening. Protect from moisture and light. No special shipping conditions or precautions are required.
Application Note
This product is a ''ready-to-use'' 1X diluent for Horseradish Peroxidase (HRP) conjugated primary or secondary. This buffer contains buffered saline along with proprietary reagents to stabilize and preserve Horseradish Peroxidase (HRP) conjugated primary or secondary antibodies in solution. A proprietary combination of preservatives is used that is azide and mercury free.
Purity/Specifity
This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry