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Supporting Reagents  >  Enzyme Substrates
 

BCIP/NBT Membrane Alkaline Phosphatase Substrate

Code

NBT-100
Size

100 mL
Price

$95.00
Availability

Ships next business day.
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Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: 5-Bromo-4-chloro-3-indonyl phosphate, X-phosphate, XP, Nitro-blue-tetrazolium chloride

BCIP/NBT Membrane Alkaline Phosphatase Substrate Properties

Description
BCIP/NBT MEMBRANE ALKALINE PHOSPHATASE SUBSTRATE - NBT-100
Application
Western Blot : 1X
Immunochemistry: 1X
Substrate/Enzyme Type
Substrate
Product Type
Substrate
Physical State
Liquid - clear to pale yellow
Shipping condition
Ambient
Label
Stabilizer
Proprietary buffer with enhancer
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BCIP/NBT Membrane Alkaline Phosphatase Substrate Description

Background
BCIP/NBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. BCIP/TNBT can be utilized in ELISA, in situ hybridization, and IHC. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.
Storage Condition
Store container at 4° C prior to opening. Protect BCIP/NBT Membrane Alkaline Phosphatase Substrate from moisture and light. No special shipping conditions or precautions are required.
Application Note
BCIP/NBT Membrane Alkaline Phosphatase Substrate is a useful detection reagent for ELISA, IHC, and in situ hybridization. The substrate is ready to use. To use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color (purple-turquise, varies with pH) will develop within 30 minutes. Wash, dry membrane and image.
Purity/Specifity
pH: 9.28 +/- 0.1 QC Raw Material: PASS Absorbance check of final product: PASS Performance Data per Immunoblot: PASS
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Custom Products;
Catalog Number
CUST13

Product Type
Molecular Biology Reagents;
Applications
ELISA
Catalog Number
MB-063-1000

Product Type
Molecular Biology Reagents;
Applications
ELISA
Catalog Number
MB-064-1000

Product Type
Supporting Reagents;
Applications
ELISA
Catalog Number
NPP-10

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry