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Supporting Reagents  >  Enzyme Substrates
 

NPP Buffer (1X Working Buffer)

Code

NPP-B500
Size

500 mL
Price

$85.00
Availability

Ships next business day.
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Shipping:
$38.00 to United States
Handling:
$20.00
Synonyms: Nitrophenylphosphate buffer, Nitrophenyl phosphate buffer, para-Nitrophenylphosphate buffer, pNPP buffer

NPP Buffer (1X Working Buffer) Properties

Description
NPP BUFFER (1X WORKING BUFFER) - NPP-B500
Application
ELISA : 50:1
Substrate/Enzyme Type
Substrate
Product Type
Substrate
Physical State
Liquid - clear
Shipping condition
Ambient
Label
Download CofA
Lot#  

NPP Buffer (1X Working Buffer) Description

Background
NPP Buffer (1x Working Buffer) is required with the use of NPP (50X) ELISA ALKALINE PHOSPHATASE SUBSTRATE, a chromogenic substrate utilized in immunosorbent assays. Phosphatase-conjugated detection antibodies can act upon the pNPP to convert it to a yellow para-nitrophenol. This yellow product can be measured on a spectrophotometer at 405nm.
Storage Condition
Store NPP Buffer (1x Working Buffer) at 4° C prior to opening. Protect NPP Buffer (1x Working Buffer) from moisture and light. No special shipping conditions or precautions are required.
Application Note
Utilized as the diluent buffer in ELISA experiments with Rockland p/n NPP-10.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Custom Products;
Catalog Number
CUST13

Product Type
Molecular Biology Reagents;
Applications
ELISA
Catalog Number
MB-063-1000

Product Type
Molecular Biology Reagents;
Applications
ELISA
Catalog Number
MB-064-1000

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry