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Kits  >  PAP Reagents for IHC
 

Rabbit Peroxidase Anti-Peroxidase (PAP) Soluble Complex

Code

P300-0025
Size

25 mg
Price

$215.00
Availability

Ships next business day.
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Synonyms: Horseradish peroxidase; HRP

Rabbit Peroxidase Anti-Peroxidase (PAP) Soluble Complex Properties

Description
RABBIT Peroxidase Anti-Peroxidase (PAP) Soluble Complex - P300-0025
Application
Immunochemistry: User Optimized
Species of Origin
Rabbit
Product Type
Component
Kit Type
PAP Kit
Physical State
Lyophilized
Shipping condition
Ambient
Concentration
20.0 mg/mL by UV absorbance at 280 nm
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Buffer
Restore with PBS + 5% normal bridging host serum
Preservative
None
Stabilizer
None
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Rabbit Peroxidase Anti-Peroxidase (PAP) Soluble Complex Description

Background
The peroxidase-antiperoxidase (PAP) approach utilizes a complex of enzyme and antibody to facilitate amplification of signal, indirectly detecting the amount of primary antibody used in an assay. Generated in Rabbit, the PAP complex can be utilized in any assay that utilizes a rabbit primary antibody. With the addition of a secondary antibody that recognizes rabbit, the reporter PAP complex allows for amplification of the luminescent signal (as three molecules of enzyme are bound to every two immunoglobulins).
Immunogen
Peroxidase (Horseradish)
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Rabbit Peroxidase Anti-Peroxidase (PAP) Soluble Complex is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Suitable for use in peroxidase enzymatic labeling experiments.
Purity/Specifity
Rabbit Peroxidase Anti-Peroxidase (PAP) Soluble Complex was prepared from rabbit polyclonal antiserum by delipidation, salt fractionation and ion exchange chromatography.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and anti-Peroxidase.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302


Product Type
Kits;
Applications
IHC
Catalog Number
P400-0025

Product Type
Kits;
Applications
IHC
Catalog Number
P500-0025

Product Label

 

Description
Peroxidase (Horseradish)
Color
Substrate dependent
Conjugation Reference
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Molecular Weight
40000
Excitation Wavelength
Conjugation Chemistry
Periodate