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Protein A DyLight™ 488 Conjugated

NCI Collaboration
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DyLight™ dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image.  DyLight™ dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods.  This image shows anti-histone detection using a DyLight™ 488 conjugate (green).  Anti-tubulin was detected using a DyLight™ 549 conjugate (red).  Nuclei were counter-stained using DAPI (blue).  The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).
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Number:
PA00-41
Size:
100 µg
Price:
$85.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

Protein A DyLight™ 488 Conjugated Properties

Description
PROTEIN A DyLight™ 488 Conjugated - PA00-41
Application
FLISA : >1:20,000
IF Microscopy : >1:5,000
Western Blot : >1:10,000
Other Dilution: User Optimized
Reagent Type
Protein A
Physical State
Lyophilized
Shipping Condition
Ambient
FP Value
3.6 moles DyLight™ 488 per mole of
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Protein A DyLight™ 488 Conjugated Description

Background
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Purity/Specifity
This product is chromatographically pure Protein A and shows predominantly a single band by SDS-PAGE.  Greater than 95% of the A280 material binds to Human IgG.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Type
Avidin, Biotin, Streptavidin;
Applications
IP
Catalog Number
PA50-00-0002

Product Label

 

Description
DyLight™ 488
Color
Green
Conjugation Reference
Modified from Thermo Fisher Scientific Inc, Rockford, IL
Molecular Weight
1011
Excitation Wavelength
493
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester