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Avidin, Biotin, Streptavidin  >  Avidin-Biotin Reagents
 

STREPTAVIDIN ALKALINE PHOSPHATASE Conjugated

Code

S000-05
Size

1 mg
Price

$175.00
Availability

Ships next business day.
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Western Blot of STREPTAVIDIN ALKALINE PHOSPHATASE Conjugated
Lane 1:  Biotin conjugated Guinea Pig IgG
Load:  50 ng per lane
Secondary antibody:  STREPTAVIDIN ALKALINE PHOSPHATASE Conjugated at 1:1,000 for 60 min at RT
Block:  MB-070 for 30 min at RT
Predicted/Obsevered Size:  28 and 55 kDa/28 and 55 kDa for Guinea Pig IgG.
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Shipping:
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Handling:
$20.00
Synonyms: ALP, Alk Phos, SA ALP,S000-05

STREPTAVIDIN ALKALINE PHOSPHATASE Conjugated Properties

Description
STREPTAVIDIN ALKALINE PHOSPHATASE Conjugated - S000-05
Application
ELISA : 1:8,000 - 1:32,000
Western Blot : 1:1,000 - 1:4,000
Immunohistochemistry: 1:200 - 1:1,000
Other Dilution: User Optimized
Reagent Type
Streptavidin
Physical State
Liquid (sterile filtered)
Shipping condition
Wet Ice
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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STREPTAVIDIN ALKALINE PHOSPHATASE Conjugated Description

Background
Streptavidin is a a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditions that would typically cause dissociation (such as organic solvents, denaturants, detergents, and extreme temperatures or pH). Streptavidin's afinity for biotin can be employed in a variety of experimental uses, from purifications to standards, to means of detection or pull down experiments. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments.
Storage Condition
Store vial at 4° C before opening. DO NOT FREEZE. Streptavidin Alkaline Phosphatase conjugated is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Application Note
Streptavidin Alkaline Phosphatase is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (NPP-10).
Purity/Specifity
Streptavidin Alkaline Phosphatase was prepared from electrophoretically pure Streptavidin.   Streptavidin Alkaline Phosphatase was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Streptavidin.  No reaction was observed against anti-Avidin.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Custom Products;
Applications
ELISA, WB, IP
Catalog Number
CUST09

Product Type
Molecular Biology Reagents;
Applications
ELISA
Catalog Number
MB-064-0100

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Type
Supporting Reagents;
Applications
ELISA
Catalog Number
NPP-10

Product Label

 

Description
Alkaline Phosphatase (Calf Intestine)
Color
Substrate dependent
Conjugation Reference
Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175, 1971.
Molecular Weight
140000
Excitation Wavelength
Conjugation Chemistry
Glutaraldehye coupling