House Phospho Banner
 
You may also want to consider these related products.
NORMAL GOAT SERUM (NGS) - B304
NORMAL GOAT SERUM (NGS) - B304
BOVINE SERUM ALBUMIN - Fraction V (Immunoglobulin and Protease Free) - BSA-50
BOVINE SERUM ALBUMIN - Fraction V (Immunoglobulin and Protease Free) - BSA-50
50X Tris-Acetate-EDTA (TAE) (2.0 M Tris-Acetate 0.05 M EDTA pH 8.3) - MB-020
50X Tris-Acetate-EDTA (TAE) (2.0 M Tris-Acetate 0.05 M EDTA pH 8.3) - MB-020
Blocking Buffer for Fluorescent Western Blotting - MB-070
Blocking Buffer for Fluorescent Western Blotting - MB-070
Avidin, Biotin, Streptavidin  >  Avidin-Biotin Reagents
 

Streptavidin Cy5 Conjugated

Code

S000-06
Size

1 mg
Price

$159.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Western Blot of Cy5 conjugated Streptavidin.  
Lane 1:  Biotin conjugated Guinea Pig IgG.  
Lane 2:  none.  
Load:  100 ng per lane.
Primary antibody:  none.
Secondary antibody:  Cy5 conjugated Streptavidin at 1:1,000 for 60 min at RT.
Block: MB-070 for 30 min at RT.
Predicted/Observed size:  55 kDa, 28 kDa for Guinea Pig IgG. 
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms:

Streptavidin Cy5 Conjugated Properties

Description
STREPTAVIDIN Cy5 Conjugated - S000-06
Application
FLISA : 1:10,000 - 1:50,000
IF Microscopy : 1:1,000 - 1:5,000
FlowCytometry : 1:500 - 1:2,500
Other Dilution: FLOW CYTOMETRY 1:500 - 1:2,500
Reagent Type
Streptavidin
Physical State
Lyophilized
Shipping condition
Ambient
Label
FP Value
10.0 moles Cy5™ per mole of
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Download CofA
Lot#  

Streptavidin Cy5 Conjugated Description

Background
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Purity/Specifity
This product was prepared from chromatographically purified streptavidin followed by conjugation to the dye Cy5 and extensive dialysis against the buffer stated above.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Streptavidin.  No reaction was observed against anti-Avidin.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
Log in to submit your product review.

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
BSA-50

Product Type
Molecular Biology Reagents;
Catalog Number
MB-020

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Cy5™
Color
Far-Red
Conjugation Reference
Modified from GE Healthcare Bio-Sciences Corp., Piscataway, NJ.
Molecular Weight
975
Excitation Wavelength
650
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester