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Supporting Reagents  >  Enzyme Substrates
 

TMB Membrane Peroxidase Substrate - Ready-To-Use

Code

TMBM-1000
Size

1 L
Price

$189.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Rockland TMB membrane substrate, chemiluminescent substrate and secondary antibodies detect rabbit primary antibodies in a variety of platforms.  Shown here is a serial 1:1 dilution of control rabbit IgG protein (011-0102, 250ng starting total load) co incubated with Rockland HRP conjugated Goat anti Rabbit IgG  (611-103-122) and Dylight 649 conjugated goat anti Rabbit (611-143-122) 1:20K in MB-070.  Blot was dried and imaged (A) on Biorad Versa Doc (30 sec, DyLight649),
(B) LiCor Odyssey Reader (700 nm),
(C )  Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec),
(D) Incubated with TMB substrate (TMBM-100) for 5 minutes and scanned, and 
(E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager.
Dot Blot of TMB Membrane Peroxidase Substrate
Antigen: A 3-fold serial dilution of Peroxidase Conjugated Secondary Rabbit IgG (p/n 611-103-122) was dotted directly to the membrane. Dilutions were run in duplicate to replicate results.
Load:  Lane 1 - 50ng Lane 2 - 16.7ng Lane 3 - 5.6ng Lane 4 - 1.9ng Lane 5 - 0.6ng
Primary antibody:  none
Secondary antibody:  none
TMBM was applied to submerge the membrane and visualize the reaction to HRP for approximately 30 seconds.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: 3,3’,5,5’-Tetramethylbenzidine; TMB-E; TMBE

TMB Membrane Peroxidase Substrate - Ready-To-Use Properties

Description
TMB MEMBRANE PEROXIDASE SUBSTRATE. READY-TO-USE - TMBM-1000
Application
Western Blot : 1X
Immunochemistry: 1X
Substrate/Enzyme Type
Substrate
Product Type
Substrate
Physical State
Liquid - clear
Shipping condition
Ambient
Label
Concentration
1X N/A
Stabilizer
Proprietary buffer with enhancer
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TMB Membrane Peroxidase Substrate - Ready-To-Use Description

Background
TMB Membrane Peroxidase Substrate (3, 3', 5, 5' - Tetramethylbenzidine) is a chromogenic substrate used to visualize antibody reactivity in ELISA experiments. In the presence of peroxidases, TMB can act as an electron donor for the conversion of peroxides to water, changing the color of solution to a blue color equivalent to the degree of reactivity. This reaction can be halted with acid to change the TMB to yellow. TMB Membrane Peroxidase Substrate is ideal for investigators in Microbiology, Immunology, and Cancer research.
Storage Condition
Store container at 4° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required.
Application Note
TMB Membrane Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB Membrane Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB Membrane Peroxidase Substrate comes ready to use.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Martin, Mufson and Mesulam. (1984). "The light side of horseradish peroxidase histochemistry". J Histochem Cytochem 32 (7): 793.
Specific Reference
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Product Type
Supporting Reagents;
Applications
ELISA
Catalog Number
TMBE-100

Product Type
Supporting Reagents;
Applications
ELISA
Catalog Number
TMBE-1000

Product Type
Supporting Reagents;
Applications
ELISA
Catalog Number
TMBE-8000

Product Type
Supporting Reagents;
Applications
WB, IHC
Catalog Number
TMBM-100

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry