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IL-35 EBI3 Antibody

Rat Monoclonal 10J811
NCI Collaboration
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Flow Cytometry of Anti-Mouse EBI3 Antibody:
Figure A: Intracellular flow cytometry of mouse EBI3 in transfected and untransfected HEK 293 cells (Brefeldin A treated, 5 hours) using mouse EBI3 antibody at 3 ug/10^6 cells.
Figure B: Intracellular flow cytometry of mouse EBI3 in stimulated and unstimulated RAW cells (LPS treated, 50 ng/ml, overnight) using mouse EBI3 antibody at 1.5 ug/10^6 cells.
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Number:
210-501-B66
Size:
100 µg
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

IL-35 EBI3 Antibody Properties

Description
Anti-Mouse EBI-3 (RAT) Monoclonal Antibody - 210-501-B66
Host
Rat
Target Species
Mouse
Known Cross Reactivity
Mouse
Clonality
Monoclonal 10J811
Application
ELISA : User Optimized
Flow Cytometry : 1.5-3.0µg/10x6 cells
Western Blot : 1µg/mL
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
EBI3
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IL-35 EBI3 Antibody Description

Background
The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-27 p28 or IL-12 p35 subunit. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R.
Synonyms
Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI-3, EBI3, IL-35, IL35
Immunogen
Anti-EBI-3 (RAT) Monoclonal Antibody was produced in rat by repeated immunizations with mature full length recombinant mouse EBI-3 produced in E.coli followed by hybridoma development.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-EBI-3 antibody has been tested for use in ELISA, Western Blot, and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse EBI3 protein. A BLAST analysis was used to suggest cross-reactivity with EBI3 from mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with EBI-3 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Lyakh L, Trinchieri G, Provezza L, Carra G, and Gerosa F. Regulation of interleukin-12/interleukin-23 production and the T-helper 17 response in humans. Immunol Rev. 226: 112-131. 2008. Pflanz S., Timans J.C., Cheung J., Rosales R., Kanzler H., Gilbert J., Hibbert L., Churakova T., Travis M., Vaisberg E., Blumenschein W.M., Mattson J.D., Wagner J.L., To W., Zurawski S., McClanahan T.K., Gorman D.M., Bazan J.F., Kastelein R.A (2002). IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naive CD4(+) T cells. Immunity 16:779-790 [PubMed: 12121660] Collison LW, Workman CJ, Kuo TT, Boyd K, Wang Y, Vignali KM, Cross R, Sehy D, Blumberg RS, and Vignali DAA. (2007) The inhibitory cytokine IL-35 contributes to regulatory T-cell function. Nature 450 (7169): 566-9. doi:10.1038/nature06306. PMID 18033300
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-B54

Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-B66

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Mouse
Catalog Number
210-401-B54

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Mouse, rat
Catalog Number
210-401-B66

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry