House TrueBlot GOC
 
You may also want to consider these related products.
MIP-3α Human Recombinant Protein - 009-001-B51
MIP-3α Human Recombinant Protein - 009-001-B51
MIP-3α Mouse Recombinant Protein - 010-001-B51
MIP-3α Mouse Recombinant Protein - 010-001-B51
Anti-RAT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 612-1302
Anti-RAT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 612-1302
BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk) - B501-0500
BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk) - B501-0500
Primary Antibodies  >  Cytokines & Growth Factor Antibodies
 

MIP-3a Antibody

Rat Monoclonal 4N5F7
Code

210-501-B51
Size

100 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Western Blot of Mouse MIP 3a (RAT) antibody  
Lane 1: mouse recombinant MIP-3a 
Lane 2: human recombinant MIP-3a 
Primary antibody: MIP 3a antibody at  0.1 ug/ml for overnight at 4°C
Secondary antibody: IRDye800™ goat anti-rat at 1:10,000 for 45 min at RT
Block: 5% BLOTTO  overnight at 4°C
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: MIP-3a , CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1

MIP-3a Antibody Properties

Description
Anti-Mouse MIP-3a (RAT) Monoclonal Antibody - 210-501-B51
Host
Rat
Target Species
Mouse
Known Cross Reactivity
mouse
Clonality
Monoclonal 4N5F7
Application
Western Blot : 0.5µg/mL
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CCL20
Download CofA
Lot#  

MIP-3a Antibody Description

Background
MIP-3a (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3a inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3a also possesses antibacterial activity against E.coli and S.aureus. MIP-3a is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
Immunogen
Anti-MIP-3a (RAT) Monoclonal Antibody was produced in rat by repeated immunizations with mature full length recombinant mouse MIP-3a produced in E.coli followed by hybridoma development.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
MIP-3a antibody has been tested for use in ELISA, IP, and Neutralization. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse MIP-3a protein. A BLAST analysis was used to suggest cross-reactivity with MIP-3a from mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with MIP-3a from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Lisignoli,G., Manferdini,C., Codeluppi,K., Piacentini,A., Grassi,F., Cattini,L., Filardo,G. and Facchini,A. (2009) CCL20/CCR6 chemokine/receptor expression in bone tissue from osteoarthritis and rheumatoid arthritis patients: different response of osteoblasts in the two groups. J. Cell. Physiol. 221 (1), 154-160. Punj,V., Matta,H., Schamus,S., Yang,T., Chang,Y. and Chaudhary,P.M. (2009) Induction of CCL20 production by Kaposi sarcoma-associated herpesvirus: role of viral FLICE inhibitory protein K13-induced NF-kappaB activation. Blood 113 (22), 5660-5668. Reboldi,A., Coisne,C., Baumjohann,D., Benvenuto,F., Bottinelli,D., Lira,S., Uccelli,A., Lanzavecchia,A., Engelhardt,B. and Sallusto,F. (2009) C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the choroid plexus is required for the initiation of EAE. Nat. Immunol. 10 (5), 514-523.
Specific Reference
Log in to submit your product review.

Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-B51

Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-B51

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Rat
Catalog Number
612-1302

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry