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Primary Antibodies  >  Sample Size Primary Antibodies
 

NOTCH 1 Antibody

Rabbit Polyclonal
Code

100-401-407S
Size

25 µL
Price

$95.00
Availability

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Immunohistochemistry of Rabbit anti-Notch1 antibody.  
Tissue:  Exocrine glands of human pancreas
Fixation: FFPE 
Primary antibody:  Notch1 antibody at 1:200 
Staining: moderate to strong membranous staining and faint to moderate cytoplasmic staining. Islets showed faint staining.
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Synonyms: Neurogenic locus Notch homolog protein 1, hN1, Translocation-associated Notch protein TAN-1.

NOTCH 1 Antibody Properties

Description
Anti-NOTCH 1 (Cleaved N terminal) (Human specific) (RABBIT) Antibody - 100-401-407S
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:20,000 - 1:60,000
Western Blot : 1:500- 1:2,000
Immunochemistry: 1:200
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
80 mg/mL by Refractometry
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
None
GeneName
NOTCH1
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Lot#  

NOTCH 1 Antibody Description

Background
Anti Notch 1 Antibody recognizes Notch 1 that is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This antibody is reactive with Notch 1 by western blotting, Immunohistochemistry (IHC) and ELISA. An 80 kDa band corresponding to Notch 1 was observed at a 1:500 dilution. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
This antiserum is directed against human NOTCH 1. Based on the immunogen sequence, we expect this antibody to react as well with mouse and rat NOTCH 1 (100% sequence homology). This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Bertrand FE, Eckfeldt CE, Lysholm AS, LeBien TW (2000) Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells. Leukemia 12: 2095-2102. Gridley T (2003) Notch signaling and inherited disease syndromes. Hum. Mol. Genet. 12: Suppl 1, R9-R13.
Specific Reference
Gupta S, Li S, Abedin Md J, Wang L, Schneider E, Najafian B, Rosenberg M. Effect of Notch activation on the regenerative response to acute renal failure. Am J Physiol Renal Physiol 298: F209–F215, 2010. Published online before print October 14, 2009, doi: 10.?1152/?ajprenal.?00451.?200 Solomon A, Mian Y, Ortega-Cava C, et al. Upregulation of the let-7 microRNA with precocious development in lin-12/Notch hypermorphic Caenorhabditis elegans mutants. Dev. Biol. 2008 Apr 15;316(2):191–199. Stylianou S, Clarke RB, Brennan K. Aberrant activation of notch signaling in human breast cancer. Cancer Res. 2006 Feb 1;66(3):1517–1525.
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
100-401-405

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, dog, chimpanzee
Catalog Number
100-401-406

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human, mouse, rat
Catalog Number
100-401-407

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
100-401-408

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry