Post Translational Modification
Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Anti-14-3-3 pS58 antibody is suitable for use in ELISA, Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 29 kDa in size corresponding to the 14-3-3 proteins phosphorylated at Ser58 in the appropriate cell lysate or extract.
Anti-14-3-3 pS58 antibody is directed against rat 14-3-3 protein phosphorylated at S58. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated 14-3-3. Minimal reactivity occurs against non-phosphorylated 14-3-3. Reactivity against 14-3-3 occurs from rat and human sources. Reactivity is expected against the following species based on 100% sequence homology: bovine, canine, chicken, mouse, non-human primates, sheep, Xenopus and zebra fish.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.