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Primary Antibodies  >  Cell Cycle Protein Antibodies
 

14-3-3 phospho S58 Antibody

Rabbit Polyclonal
Code

600-401-D02
Size

100 µL
Price

$309.00
Availability

Ships within 2 weeks.
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Western Blot of Rabbit  Anti-14-3-3 pS58 Antibody.  
Lane 1: rat brainstem lysate.  
Lane 2:  rat brainstem lysate blocked by the phosphopeptide.  
Load:  10 µg per lane.
Primary antibody:  14-3-3 pS58 antibody at 1:400 for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~29kDa, ~29kDa for 14-3-3 protein phosphorylated at Ser58. 
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$20.00 to United States
Handling:
$20.00
Synonyms: 14-3-3 protein beta/alpha, Prepronerve growth factor RNH-1, Protein kinase C inhibitor protein 1

14-3-3 phospho S58 Antibody Properties

Description
Anti-14-3-3 pS58 (Rabbit) Antibody - 600-401-D02
Host
Rabbit
Target Species
Rat
Known Cross Reactivity
rat, human
Clonality
Polyclonal
Application
ELISA : 1:10,000
Western Blot : 1:1000
Immunohistochemistry: 1:500
Physical State
Liquid
Shipping condition
Dry Ice
Format
IgG
Label
Buffer
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
GeneName
YWHAB
Related Links
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Lot#  

14-3-3 phospho S58 Antibody Description

Background
14-3-3 pS58 antibody detects 14-3-3 protein phosphorylated at S58. 14-3-3 is a member of a family of highly conserved proteins that appear to have multiple roles in cell signaling. 14-3-3 is abundantly expressed in the brain and has been detected in the cerebrospinal fluid of patients with different neurological disorders. 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms. 14-3-3 affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation. Anti-14-3-3 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Immunogen
Anti-14-3-3 pS58 antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Application Note
Anti-14-3-3 pS58 antibody is suitable for use in ELISA, Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 29 kDa in size corresponding to the 14-3-3 proteins phosphorylated at Ser58 in the appropriate cell lysate or extract.
Purity/Specifity
Anti-14-3-3 pS58 antibody is directed against rat 14-3-3 protein phosphorylated at S58. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated 14-3-3. Minimal reactivity occurs against non-phosphorylated 14-3-3. Reactivity against 14-3-3 occurs from rat and human sources. Reactivity is expected against the following species based on 100% sequence homology: bovine, canine, chicken, mouse, non-human primates, sheep, Xenopus and zebra fish.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Berg D, Holzmann C and Riess O (2003). 14-3-3 Proteins in the nervous system. Nat Rev Neurosci 4:752-762. Bridges D and Moorhead GB (2005). 14-3-3 Proteins: a number of functions for a numbered protein. Sci STKE 2005:re10. Dougherty MK and Morrison DK (2004). Unlocking the code of 14-3-3. J Cell Sci 117:1875-1884. Silhan J, Obsilova V, Vecer J, Herman P, Sulc M, Teisinger J and Obsil T (2004). 14-3-3 Protein C-terminal stretch occupies ligand binding groove and is displaced by phosphopeptide binding. J Biol Chem 279:49113-49119. Woodcock JM, Murphy J, Stomski FC, Berndt MC and Lopez AF (2003). The dimeric versus monomeric status of 14-3-3 zeta is controlled by phosphorylation of Ser58 at the dimer interface. J Biol Chem 278:36323-36327. Yang X, Luo C, Cai J, Pierce W and Tezel G (2008). Phosphorylation-Dependent Interaction with 14-3-3 in the Regulation of Bad Trafficking in Retinal Ganglion Cells. Invest. Ophthalmol. Vis. Sci., 49: 2483 - 2494.
Specific Reference
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Rating
Name
Application
Species
Submitted On
ELISA
Rabbit
11/27/2012 10:17:27 AM
Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
600-401-281

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302


Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry