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Beta Catenin phospho S33/phospho S37 Antibody

Rabbit Polyclonal
NCI Collaboration
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Western Blot of Rabbit Anti-beta Catenin pS33/pS37  antibody.  
Lane 1:  human embryonic kidney cell (HEK) lysate.  
Lane 2: human embryonic kidney cell (HEK) lysate with glycogen synthase kinase 3ß knocked down by SiRNA.  
Load:  10 µg per lane.
Upper Panel: ß-catenin phosphorylated at Ser33 and Ser37.
Lower Panel: pan ß-catenin.
Primary antibody:  ß-catenin pS33 and S37 antibody at 1:400 for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  ~83 kDa, ~83 kDa for ß-catenin pS33 and S37. 
Other band(s): none.
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Number:
609-401-D12
Size:
100 µL
Price:
$319.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships within 2 weeks.

Beta Catenin phospho S33/phospho S37 Antibody Properties

Description
Anti-beta Catenin pS33/pS37 (Rabbit) Antibody - 609-401-D12
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:10,000
Western Blot : 1:1,000
Physical State
Liquid
Shipping Condition
Dry Ice
Format
IgG
Label
Buffer
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
GeneName
CTNNB1
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Beta Catenin phospho S33/phospho S37 Antibody Description

Background
Beta Catenin antibody recognizes ß-catenin which is a central component of the cadherin cell adhesion complex and plays an essential role in neural development in the Wingless/Wnt signaling pathway. The role of ß-catenin in these processes is thought regulated by the sequential phosphorylation of Ser29 Ser33, Ser37 and Thr41 by glycogen synthase kinase 3ß (GSK3ß). This hyperphosphorylation promotes the ubiquitylation and targeted destruction of ß-catenin. Mutations in components of this phosphorylation regulated process that prevent ß-catenin hyperphosphorylation by GSK3ß are trongly associated with cancers. Anti-Beta Catenin antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Synonyms
Catenin beta-1, beta catenin, ß-catenin, CTNNB1, CTNNB, beta catenin antibody, anti-beta catenin
Immunogen
Anti-Beta Catenin pS33/pS37 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser33/37.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Application Note
Anti-beta Catenin pS33/pS37 antibody is suitable for use in ELISA, Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 83 kDa in size corresponding to the beta catenin protein phosphorylated at Ser33 and 37 in the appropriate cell lysate or extract.
Purity/Specifity
Anti-Beta Catenin pS33/pS37 Antibody is directed against beta human Catenin protein phosphorylated ar S33/pS37. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated beta Catenin. Minimal reactivity occurs against non-phosphorylated beta Catenin. Reactivity against beta Catenin occurs from human sources. However, reactivity is also expected against mouse, rat and Xenopus based on 100% sequence homology. Cross reactivity from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Ding Y and Dale, T (2002). Wnt signal transduction: kinase cogs in a nano-machine? Trends Biochem Sci 27:327-329. Liu C, Li Y, Semenov M, Han C, Baeg GH, Tan Y, Zhang Z, Lin X and He X (2002). Control of ß-catenin phosphorylation/degradation by a dual-kinase mechanism. Cell 108:837-847. Polakis P (2000). Wnt signaling and cancer. Genes Dev 14:1837-1851. Wang ZH, Vogelstein B and Kinzler KW (2003). Phosphorylation of ß-catenin at S33, S37, or T41 can occur in the absence of phosphorylation at T45 in colon cancer cells. Cancer Res 63:5234-5235.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
200-301-A37


Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry