House TrueBlot GOC
 
You may also want to consider these related products.
Anti-cdk5 (RABBIT) Antibody - 100-401-163
Anti-cdk5 (RABBIT) Antibody - 100-401-163
Anti-RABBIT IgG (H&L) (GOAT) Antibody Texas Red™ Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) - 611-109-122
Anti-RABBIT IgG (H&L) (GOAT) Antibody Texas Red™ Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) - 611-109-122
2X SDS-PAGE Sample Buffer without DTT or b-MeOH (125 mM Tris HCl, 20% Glycerol, 4% SDS, 0.1% bromphenol blue, pH 6.8) - MB-018
2X SDS-PAGE Sample Buffer without DTT or b-MeOH (125 mM Tris HCl, 20% Glycerol, 4% SDS, 0.1% bromphenol blue, pH 6.8) - MB-018
Blocking Buffer for Fluorescent Western Blotting - MB-070
Blocking Buffer for Fluorescent Western Blotting - MB-070
Primary Antibodies  >  Phospho Specific Antibodies
 

GABA(A) Receptor gamma 2 phospho S327 Antibody

Rabbit Polyclonal
Code

612-401-D52
Size

100 µL
Price

$309.00
Availability

Ships within 2 weeks.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Western Blot of Rabbit Anti-GABA(A) Receptor gamma 2 pS327 antibody.  
Lane 1:  rat cortex.  
Lane 2:  rat cortex blocked by the phospho-peptide.  
Load:  10 µg per lane.
Primary antibody:  GABAA-R antibody at 1:400 for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  ~45kDa/~45kDa for GABAA ? 2 protein phosphorylated at Ser327. 
Other band(s): none.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Gamma-aminobutyric acid receptor subunit gamma-2, GABA(A) receptor subunit gamma-2, Gabrg2

GABA(A) Receptor gamma 2 phospho S327 Antibody Properties

Description
Anti-GABA(A) Receptor gamma 2 pS327 (Rabbit) Antibody - 612-401-D52
Host
Rabbit
Target Species
Rat
Known Cross Reactivity
rat
Clonality
Polyclonal
Application
Western Blot : 1:1000
Physical State
Liquid
Shipping condition
Dry Ice
Format
IgG
Label
Buffer
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
GeneName
GABRG2
Download CofA
Lot#  

GABA(A) Receptor gamma 2 phospho S327 Antibody Description

Background
Anti-GABA(A) Receptor gamma 2 pS327 Antibody detects phosphorylated GABA(A) Receptor gamma 2. Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six as, four ßs and four ?s, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for a- and ß-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a ?-subunit is required for benzodiazepine modulation. It has recently been suggested that PKC? regulates the sensitivity of GABAA a1ß2?2 receptors to ethanol and benzodiazepines through phosphorylation of serine 327 in the large intracellular loop of ?2. GABA(A) Receptor gamma 2 pS327 antibody is ideal for investigators involved in Neuroscience.
Immunogen
Anti-GABA(A) Receptor gamma 2 pS327 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Application Note
Anti-GABA(A) Receptor gamma 2 pS327 (Rabbit) antibody is suitable for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 45 kDa in size corresponding to GABA(A) receptor gamma 2 subunit phosphorylated at Ser327 in the appropriate cell lysate or extract.
Purity/Specifity
Anti-GABA(A) Receptor gamma 2 pS327 Antibody is directed against rat phosphorylated GABA(A) Receptor gamma 2. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Immunolabeling of the GABAA band is completely blocked by ?-phosphatase treatment. This antibody is directed against rat GABA(A) receptor gamma 2 proteins phosphorylated at Ser327. Cross reactivity with GABA(A) Receptor gamma 2 pS327 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Olsen RW and Tobin AJ (1990). Molecular biology of GABAA receptors. FASEB 4:1469-1480. Whiting PJ, Bonnert TP, McKernan RM, Farrar S, Le Bourdellès B, Heavens RP, Smith DW, Hewson L, Rigby MR, Sirinathsinghji DJS, Thompson SA and Wafford KA (1999). Molecular and functional diversity of the expanding GABAA receptor gene family. Ann NY Acad Sci 868:645-653. Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W and Sieghart W (2004). Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor ?2-subunit. Biochem Pharmacol 68:1621-1629. Qi ZH, Song M, Wallace MJ, Wang D, Newton PM, McMahon T, Chou WH, Zhang C, Shokat KM and Messing RO (2007). Protein kinase Ce regulates ?-aminobutyrate type A receptor sensitivity to ethanol and benzodiazepines through phosphorylation of ?2 subunits. J Biol Chem 282(45):33052-63.
Specific Reference
Log in to submit your product review.

Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
human, rat and mouse
Catalog Number
100-401-163



Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry