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Primary Antibodies  >  Phospho Specific Antibodies
 

NMDA R2B phospho T1252 Antibody

Rabbit Polyclonal
Code

612-401-D92
Size

100 µL
Price

$309.00
Availability

Ships within 2 weeks.
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Western Blot of Rabbit anti-NMDA R2B pT1252 antibody.  
Lane 1:  rat hippocampal lysate (control).  
Lane 2:  ?-Ptase.  
Load:  10 µg per lane.
Primary antibody:  NMDA R2B pT1252 antibody at 1:1,000 for overnight at 4°C.
Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  180 kDa for NMDA R2B pT1252. 
Other band(s): NMDA R2B pT1252 splice variants and isoforms.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B, Grin2b

NMDA R2B phospho T1252 Antibody Properties

Description
Anti-NMDA R2B pT1252 (Rabbit) Antibody - 612-401-D92
Host
Rabbit
Target Species
Rat
Known Cross Reactivity
rat
Clonality
Polyclonal
Application
Western Blot : 1:1000
Immunochemistry: 1:400
Other Dilution: User Optimized
Physical State
Liquid
Shipping condition
Dry Ice
Format
IgG
Label
Buffer
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
GeneName
GRIN2B
Download CofA
Lot#  

NMDA R2B phospho T1252 Antibody Description

Background
NMDA R2B pT1252 Antibody detects NMDA R2B protein. The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death. The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Phosphorylation of Tyr1252 is thought to potentiate NMDA receptor-dependent influx of calcium. Anti-NMDA R2B pT1252 Antibody is ideal for investigators involved in Neuroscience, Signal Transduction, and Cell Signaling Research.
Immunogen
Anti-NMDA R2B pT1252 Antibody was producedby repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1252.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Application Note
Anti-NMDA R2B pT1252 Antibody is suitable for use in Western Blotting and IHC. Expect a band of approximately 180 kDa in size corresponding to NMDAR NR2B subunit protein phosphorylated at Tyr1252 in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-NMDA R2B pT1252 antibody is directed against NMDA R2B protein. NMDA R2B pT1252 antibody was affinity purified from monospecific antiserum by immunoaffinity purification. The antibody is specific for the phophorylated NMDA R2B protein. Immunolabeling of the NMDA NR2B subunit band is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. Immunolabeling is also blocked by ?-phosphatase treatment. The antibody may also show some slight reactivity with Tyr1246 of NR2A. Reactivity is expected from the following species based on 100% sequence homology: bovine, canine, chicken, human, mouse, non-human primate and zebra fish.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Carroll RC and Zukin RS (2002). NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577. Grosshans DR, Clayton DA, Coultrap SJ and Browning MD (2002). LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33. Ishii T, Moriyoshi K, Sugihara H, Sakurada K, Kadotani H, Yokoi M, Akazawa C, Shigemoto R, Mizuno N, Masu M and Nakanishi S (1993). Molecular characterization of the family of the N-methyl- D-aspartate receptor subunits. J Biol Chem 268:2836-2843. Takasu, MA, Dalva, MB, Zigmond, RE and Greenberg, ME (2002). Modulation of NMDA Receptor -Dependent Calcium Influx and Gene Expression Through EphB Receptors. Science 295:491-495. Wenthold RJ, Prybylowski K, Standley S, Sans N and Petralia RS (2003). Trafficking of NMDA receptors. Annu Rev Pharmacol Toxicol 43:335-358. Hicklin TR, Wu PH, Radcliffe RA, Freund RK, Goebel-Goody SM, Correa PR, Proctor WR, Lombroso PJ and Browning MD (2011). Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase PNAS, Apr 2011; 108: 6650 - 6655.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat, dog, orangutan
Catalog Number
600-401-873

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-106-122


Product Type
Avidin, Biotin, Streptavidin;
Applications
ELISA, WB, IHC
Catalog Number
S000-03

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry