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Primary Antibodies  >  Sample Size Primary Antibodies
 

Histone H2AvD pS137 Antibody

Rabbit Polyclonal
Code

600-401-914S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
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Immunohistochemistry with anti-Histone Antibody.
Tissue:  Human Bladder Cancer.
Fixation:  FFPE buffered formalin 10% conc.
Ag Retrieval: HIER  citrate buffer pH6  or HIER  EDTA  pH9.
Primary antibody:  2 ug/ml at 2 hr. 
Secondary Ab:  anti  rabbit polymer HRP 20 ‘ RT.
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Shipping:
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$20.00
Synonyms: H2AvD protein antibody

Histone H2AvD pS137 Antibody Properties

Description
Anti-Histone H2AvD pS137 (RABBIT) Antibody - 600-401-914S
Host
Rabbit
Target Species
Drosophila melanogaster
Known Cross Reactivity
Drosophila melanogaster
Clonality
Polyclonal
Application
ELISA : 1:4,000 - 1:16,000
Western Blot : 1:400 - 1:1,600
Immunochemistry: 2 ug/ml
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
HIS2AV
Related Links
Download CofA
Lot#  

Histone H2AvD pS137 Antibody Description

Background
Variant histones H2A are synthesized throughout the cell cycle and are very different from classical S-phase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known. H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4. The octamer wraps approximately 146 bp of DNA. HsAvD is expressed both maternally and zygotically and is found in embryos through to adults (female only). The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur. In mouse, H2AX ''knock out'' mice have an increased incidence of cancer.
Immunogen
Histone H2AvD pS137 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 132-141 of Drosophila melanogaster (fruit fly) H2AvD protein.
Immunogen Type
Peptide
Post Translational Modification
Phosphorylation
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
Histone H2AvD pS137 Antibody has been tested for use in ELISA, Immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 14 kDa in size corresponding to phosphorylated H2AvD protein by western blotting in the appropriate Drosophila tissue or cell lysate or extract. Less than 0.2% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS137 of H2AvD protein.
Purity/Specifity
This affinity purified anti-Histone H2AvD pS137 Antibody is directed against the phosphorylated form of Drosophila H2AvD protein at the pS137 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against Drosophila H2AvD pS137 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA. A BLAST analysis was used to suggest little to no cross reactivity with H2AvD proteins from other sources based on a comparison using the immunizing sequence. Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Mason,J.M., Ransom,J. and Konev,A.Y. (2004) A deficiency screen for dominant suppressors of telomeric silencing in Drosophila. Genetics 168 (3), 1353-1370. Kimura,K., Kodama,A., Hayasaka,Y. and Ohta,T. (2004) Activation of the cAMP/PKA signaling pathway is required for post-ecdysial cell death in wing epidermal cells of Drosophila melanogaster. Development 131 (7), 1597-1606. Madigan, J.P., Chotkowski, H.L. and Glaser, R.L. (2002) DNA double strand breaks-induced phosphorylation of Drosophila histone variant H2Av helps prevent radiation-induced apoptosis. Nucleic Acid Res. 30:17; 368-3705.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat
Catalog Number
200-301-400

Product Type
Secondary Antibodies;
Applications
FLISA, IF, FC
Catalog Number
211-1202

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human
Catalog Number
600-401-879

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Drosophila melanogaster
Catalog Number
600-401-914

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry