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Primary Antibodies  >  Sample Size Primary Antibodies

COLLAGEN Type I Antibody

Rabbit Polyclonal


25 µL


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Rockland's Affinity Purified anti-Collagen I antibody was used at a 1:100 dilution to detect distal tubules in normal kidney tissue.  Note the absence of staining of glomeruli.  The antibody was reacted with antibody for 4 hours at room temperature followed by the addition of secondary antibody and substrate reaction.  Tissue was formalin-fixed and paraffin embedded.  No antigen retrieval was performed.
Western blot of Human Collagen Type I.
Lane 1:  Human Collagen Type 1.
Lane 2:  None.
Load:  50 ng per lane.
Primary antibody:  Collagen Type I antibody at 1:1,000 overnight at 4°C.
Secondary antibody:  DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT.
Block:  MB-070 for 30 min at RT.
Predicted/Observed size:  139 & 130 kDa, 139 & 130 kDa for Collagen Type I.
Other Band(s):  Collagen Type I splice variants and isoforms.
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$20.00 to United States
Synonyms: Collagen Of Skin Tendon And Bone antibody, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen type I alpha 2 antibody, OI4 antibody, Osteogenesis Imperfecta Type IV antibody

COLLAGEN Type I Antibody Properties

Anti-Collagen Type I (RABBIT) Antibody - 600-401-103S
Target Species
Known Cross Reactivity
human, mouse, rat, bovine
ELISA : 1:5,000 - 1:50,000
Western Blot : 1:1,000 - 1:10,000
Immunohistochemistry: 1:50 - 1:200
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
1.0 mg/mL by UV absorbance at 280 nm
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
0.01% (w/v) Sodium Azide
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COLLAGEN Type I Antibody Description

Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.
Collagen Type I from human and bovine placenta
Immunogen Type
Native Protein
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis.
COLLAGEN I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
Stefanovic, B, Schnabl, B, Brenner, DA (2002) Inhibition of collagen alpha 1(I) expression by the 5' stem-loop as a molecular decoy. J.Biol. Chem. 277(20):18229-18237. Hashimoto, N et al. (2004) Bone marrow-derived progenitor cells in pulmonary fibrosis. J. Clin. Invest. 113:243-252. Hazra, S et al. (2004) Peroxisome Proliferator-activated Receptor gamma Induces a Phenotypic Switch from Activated to Quiescent Hepatic Stellate Cells. J. Biol. Chem. 279(12):11392–11401. She, H, Xiong, S, Hazra, S, Tsukamoto, H (2005) Adipogenic transcriptional regulation of hepatic stellate cells. J. Biol. Chem. 280(6):4959-4967. Mak KM, Kwong AJ, Chu E, Hoo NM. (2011) Hepatic Steatosis, Fibrosis, and Cancer in Elderly Cadavers. Cancer Biology. 5 DEC 2011. DOI: 10.1002/ar.21525.
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Product Type
Proteins and Peptides;
Catalog Number

Product Type
Proteins and Peptides;
Reacts With
Catalog Number

Product Type
Primary Antibodies;
Reacts With
human, mouse, rat, bovine
Catalog Number

Product Type
Primary Antibodies;
Reacts With
human, mouse, rat, bovine
Catalog Number

Product Label


Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry