Western Blot of Rabbit Anti-IL-6 Antibody

Datasheet

IL-6 Antibody

Name: IL-6 Antibody
Brand: Rockland Immunochemicals
Price: 1225.0 USD
Availability: InStock

Rabbit Polyclonal

2 References

Item No. 109-401-310

Size: 1 mL

Applications: WB, IHC, Dot Blot

Reactivity: Human

Host Species: Rabbit

$1,225.00 /Per Item

25 µL $125.00

1 mL $1,225.00

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Background

Anti-IL-6 Antibody recognizes human IL-6 that is a secreted cytokine with a wide variety of biological functions. IL-6 is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Anti-IL6 antibody is ideal for investigators involved in Immunology and Cancer research.

Expand Background

Specifications for IL-6 Antibody

Product Details

Description: Anti-Human IL-6 (RABBIT) Antibody - 109-401-310

Synonyms: HSF, Hybridoma growth factor, HGF, Hybridoma plasmacytoma growth factor, IL 6, IL6 protein, IFNB2, Interferon beta 2, BSF2, CDF, B-Cell Differentiation Factor, B-cell stimulatory factor 2, CTL differentiation factor, rabbit anti-Interleukin 6 Antibody, rabbit anti-IL-6

Host Species: Rabbit

Clonality: Polyclonal

Format: Antiserum

Target Details

Gene Name: IL6 - View All IL6 Products

Reactivity: Human

Immunogen Type: Recombinant Protein

Immunogen: This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli.

Purity/Specificity: Anti-IL-6 antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). This product has minimal reactivity with mouse IL-6.

Database Links:

UniProtKB - P05231

NCBI - NP_000591.1

GeneID - 3569

Application Details

Tested Applications: Dot Blot, WB

Suggested Applications: IHC - View References

Application Note: IL-6 antibody has been tested in western blot. This antibody is suitable for use in neutralizations, ELISA, radioimmunoassay, and immunoprecipitation. Reactivity in other immunoassays is unknown. Expect ~23.7kDa. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. For immunoblot use the supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10ng/mL E. coli LPS. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. The antiserum is useful for neutralization of human of IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 80 mg/mL by Refractometry

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: None

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (2)

Asano S et al. (2020). Preliminary report: the relevance of tumor necrosis factor-α in acquired primary lymphedema—a histopathological investigation. Lympat Res Biol.

Applications

IHC, ICC, Histology

PubMed

Kanbe, K et al. (2009). Inducement of mitogen-activated protein kinases in frozen shoulders. Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association

Applications

IHC, ICC, Histology

PubMed

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

General Immunoblotting Protocol

Heat-induced Antigen Retrieval Protocol

Histone Immunoblotting Protocol

Immunohistochemistry (IHC) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Protease-induced Antigen Retrieval Protocol

Staining Paraffin Sections by PAP Procedure

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.