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GFP and Epitopes  >  Antibodies to GFP and Antibodies to RFP
 

GFP Antibody

Goat Polyclonal
Code

600-101-215S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
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Immunofluorescence Microscopy of GFP-GOAT-Antibody.  
Tissue:  Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification).  
Fixation:  4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution.
Antigen retrieval:  frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns.
Primary antibody: Goat anti-GFP was used at 1:500 dilution in free floating imunnohistochemistry to detect GFP.
Secondary antibody:  Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1:10,000 for 45 min at RT.
Localization:  Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus.
Staining:  eGFP as green fluorescent signal and sections were counterstained with DAPI.
Immunofluorescence Microscopy of GFP-GOAT Antibody.  
Tissue:  Drosophila melanogaster late stage embryonic central nervous system.  
Fixation:  0.5% PFA. 
Antigen retrieval:  not required.
Primary antibody:  Anti-GFP antibody at a 1:1,000 for 1 h at RT.
Secondary antibody:  AlexaFluor 488™ conjugated Anti-Goat antibody at 1:300 for 45 min at RT.
Panel A: shows a lateral view (ventral left).  
Panels B and C: shows ventral views of whole mount embryos at 63x magnification (plus 2x digital zoom). 
In all panels, anterior is up.
Staining:  tau-GFP cell bodies (large arrowhead) and axons of motorneurons (arrow) and interneurons (small arrowhead) as green fluorescent signal.
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Shipping:
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Handling:
$20.00
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.

GFP Antibody Properties

Description
Anti-GFP (GOAT) Antibody - 600-101-215S
Host
Goat
Known Cross Reactivity
wt, rGFP, eGFP
Clonality
Polyclonal
Application
ELISA : 1:40,000
Western Blot : 1:400 - 1:2,000
Immunochemistry: 1:200 - 1:1,000
Other Dilution: User Optimized
Epitope Tag Type
GFP
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
Download CofA
Lot#  

GFP Antibody Description

Background
Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Immunogen
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
Anti-GFP is designed to detect GFP and its variants. Goat Anti-GFP is ideal for western blotting, ELISA, Immunohistochemistry and IP. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with Rockland's monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin-HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and detects GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP-containing proteins on western blots. Researchers should determine optimal titers for applications.
Purity/Specifity
GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Phillips G., (2001) Green fluorescent protein--a bright idea for the study of bacterial protein localization. FEMS Microbiol Lett 204 (1): 9–18. doi:10.1016/S0378-1097(01)00358-5. PMID
Specific Reference
Kofidis, T., de Bruin, J. L., Yamane, T., Tanaka, M., Lebl, D. R., Swijnenburg, R. J., ... & Robbins, R. C. (2005). Stimulation of paracrine pathways with growth factors enhances embryonic stem cell engraftment and host-specific differentiation in the heart after ischemic myocardial injury. Circulation, 111(19), 2486-2493. Post, H., Kajstura, J., Lei, B., Sessa, W. C., Byrne, B., Anversa, P., ... & Recchia, F. A. (2003). Adeno-associated virus mediated gene delivery into coronary microvessels of chronically instrumented dogs. Journal of Applied Physiology, 95(4), 1688-1694. Hashimoto, N., Jin, H., Liu, T., Chensue, S. W., & Phan, S. H. (2004). Bone marrow–derived progenitor cells in pulmonary fibrosis. Journal of Clinical Investigation, 113(2), 243-252.
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC, IF, IP
Reacts With
wt, rGFP, eGFP
Catalog Number
600-101-215

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry