House Buffer
 
You may also want to consider these related products.
TNF-α Human Recombinant Protein - 009-001-306
TNF-α Human Recombinant Protein - 009-001-306
TNF-α Mouse Recombinant Protein - 010-001-321
TNF-α Mouse Recombinant Protein - 010-001-321
Anti-Human TNF-α (RABBIT) Antibody - 209-401-306
Anti-Human TNF-α (RABBIT) Antibody - 209-401-306
Anti-Swine TNFα (RABBIT) Antibody - 214-401-306S
Anti-Swine TNFα (RABBIT) Antibody - 214-401-306S
Primary Antibodies  >  Sample Size Primary Antibodies
 

TNF alpha Antibody

Rabbit Polyclonal
Code

209-401-306S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Immunohistochemistry using Rockland's polyclonal TNFa antibody showing staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections.  Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum.  Primary antibody was diluted 1:100 and incubated with  samples for 24 hours at 4°C.  HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.
Western blot using Rockland's Anti-Human TNF-a (RABBIT) Antibody. Membrane blocked in 1% BSA-TBS-T 30 min RT, Rb-a-TNF alpha added at 1:1000 in 1% BSA-TBS-T o/n 4°C, DyLight 649 Gt-a-Rb 611-143-122 added at 1:20,000 in MB-070 30 min RT.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody

TNF alpha Antibody Properties

Description
Anti-Human TNF-a (RABBIT) Antibody - 209-401-306S
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human
Clonality
Polyclonal
Application
ELISA : 1:1,000 - 1:5,000
Western Blot : 1:500 - 1:2,000
Immunohistochemistry: 1:100 - 1:500
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
None
Stabilizer
None
GeneName
TNFA
Related Links
Download CofA
Lot#  

TNF alpha Antibody Description

Background
Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-a) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
Immunogen
The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFa.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
This IgG fraction antibody of anti-Human TNFa has been tested for use in neutralizations, ELISA, immunohistochemistry and immunoblotting. It recognizes the 17,000 MW TNFa. Reactivity in other immunoassays is unknown.
Purity/Specifity
This antibody is primarily directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNGß (lymphotoxin). This IgG fraction antibody will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Kant S, Swat W, Zhang S, Zhang ZY, Neel BG, Flavell RA, Davis RJ (2011). TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway. Genes Dev 25 (19): 2069–78. doi:10.1101/gad.17224711. PMC 3197205. PMID 21979919
Specific Reference
Log in to submit your product review.

Product Type
Proteins and Peptides;
Reacts With
Human
Catalog Number
009-001-306

Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-321

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
209-401-306

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
Swine
Catalog Number
214-401-306S

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry