You may also want to consider these related products.

Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody - 200-301-880

View Product.

Anti-alpha-Tubulin (RABBIT) Antibody - 600-401-880

View Product.

Anti-beta Actin (RABBIT) Antibody - 600-401-886

View Product.

Anti-beta Actin (RABBIT) Antibody Peroxidase Conjugated - 600-403-886

View Product.

Primary Antibodies > Sample Size Primary Antibodies

Nestin Antibody

Rabbit Polyclonal

Code

600-401-417S

Size

25 µL

Price

$89.00

Availability

Ships next business day.

Order Offline:

Phone: 800.656.7625

E-mail: orders@rockland-inc.com

Fax: 610.367.7825

Bulk Orders:

Call or E-mail Us For Information
Fill out our Contact Form

Immunohistochemistry with Anti-nestin antibody
Tissue: mouse kidney (Left) and human kidney (Right).
Fixation: formalin-fixed, paraffin-embedded tissue
Antigen retrieval: heat-induced
Primary antibody: 5 µg/ml
Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Shipping & Handling Fees Apply.

Shipping:

$85.00 to United States

Handling:

$45.00

Synonyms: nestin, ESTM 46 antibody, FLJ 21841 antibody, FLJ21841 antibody, Intermediate filament protein antibody

Datasheet
References
Customer Reviews
Protocols
Related Products

Nestin Antibody Properties

Description

Anti-Nestin (RABBIT) Antibody - 600-401-417S

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human

Clonality

Polyclonal

Application

ELISA : 1:20,000 - 1:80,000
Western Blot : 1:500 - 1:3,000
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.1 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

NES

Nestin Antibody Description

Background

Nestin, is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985). Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1484-1500 of human Nestin protein.

Immunogen Type

Recombinant Protein

Storage Condition

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Application Note

This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 200-220 kDa in size corresponding to Nestin protein by western blotting in the appropriate cell lysate or extract.

Purity/Specifity

This affinity purified antibody is directed against human Nestin protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Nestin protein from human and macaque (100% homology). Based on protein sequence homology also expect partial reactivity against Nestin homologues from rabbit (94%), mouse (reactivity shown above), rat and hamster (88%), and bovine and dog (82%). Reactivity against homologues from other sources is not known.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Dahlstrand,J., Zimmerman,L.B., McKay,R.D. and Lendahl,U. (1992) Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments. J. Cell. Sci. 103 (Pt 2), 589-597. Yaworsky,P.J. and Kappen,C. (1999) Heterogeneity of neural progenitor cells revealed by enhancers in the nestin gene. Dev. Biol. 205 (2), 309-321. Hockfield,S. and McKay,R.D. (1985) Identification of major cell classes in the developing mammalian nervous system. J. Neurosci. 5 (12), 3310-3328. Mokry,J., Cizkova,D., Filip,S., Ehrmann,J., Osterreicher,J., Kolar,Z. and English,D. (2004) Nestin expression by newly formed human blood vessels. Acta Neurochir. Suppl. 13 (6), 658-664. Aihara,M., Sugawara,K., Torii,S., Hosaka,M., Kurihara,H., Saito,N. and Takeuchi,T. (2004) Angiogenic endothelium-specific nestin expression is enhanced by the first intron of the nestin gene. Lab. Invest. 84 (12), 1581-1592.

Specific Reference

There are no reviews for this product. Log in to submit your own review.

AMCA Conjugates

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra