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Avidin, Biotin, Streptavidin  >  Avidin-Biotin Reagents
 

SEPHAROSE™ PROTEIN A/G

Code

PAG50-00-0002
Size

2 mL
Price

$69.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
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Sepharose™ Protein A/G is a suspension of beads conjugated to a fusion protein containing binding domains from both protein A (from Staphylococcus aureaus) and protein G (from Streptococcus sp.).
Ideal for Western Blotting where photo sensitive Conjugated Antibodies such as IRDye Conjugated Antibodies and DyLight Conjugated Antibodies are used. Rockland Immunochemicals features both large and small boxes to fit varying size and volume requirements.
Shipping & Handling Fees Apply.

Shipping:
$70.00 to United States
Handling:
$20.00
Synonyms: Sepharose Protein A/G, agarose, immunoprecipitation, IP western blot

SEPHAROSE™ PROTEIN A/G Properties

Description
SEPHAROSE™ PROTEIN A/G - PAG50-00-0002
Application
Western Blot : User Optimized
ImmunoPrecipitation: User optimized
Other Dilution: User Optimized
Physical State
Suspension of agarose beads
Shipping condition
Wet Ice
PSC Format
Agarose Conjugate
Label
Buffer
20% (v/v) Ethanol
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Lot#  

SEPHAROSE™ PROTEIN A/G Description

Background
Sepharose™ Protein A/G can be used for immunoprecipitation and purification of monoclonal antibodies. Sepharose™ Protein A/G is a suspension of beads conjugated to a fusion protein containing binding domains from both protein A (from Staphylococcus aureaus) and protein G (from Streptococcus sp.). When evenly dispersed approximately 2.0 ml of suspension is equal to 0.5 cc of settled beads of Sepharose™ 4 Fast Flow (highly cross linked, 4% agarose derivative). The bead size is approximately 45-165 µm when swollen. Each cc of drained beads will bind approximately 20-30 mg Human IgG. The exclusion limit (Mr) of the beads is 3 x 10^7. The maximum linear flow rate is approximately 1300 cm/hr. This product shows long term stability within pH range of 3 to 9 and short term stability within pH range of 2 to 10. Up to 100 reactions.
Storage Condition
Store SEPHAROSE PROTEIN A/G at 4° C prior to opening. DO NOT FREEZE.
Application Note
Protein A/G resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.
Purity/Specifity
The product can be used for binding IgG from human, rabbit, goat, mouse and rat. It has strong binding of human, rabbit, cow, sheep, and goat polyclonal antibodies, mouse IgG2a, IgG2b, IgG3, and rat IgG2a. Protein A/G also has moderate affinity for mouse and rat IgG1 and IgG2c. It binds with weak affinity to rat IgG2b. Sepharose™ Protein A/G can be used for immunoprecipitation and purification of monoclonal antibodies.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Molecular Biology Reagents;
Catalog Number
MB-001

Product Type
Molecular Biology Reagents;
Catalog Number
MB-003

Product Type
Molecular Biology Reagents;
Catalog Number
MB-008

Product Type
Molecular Biology Reagents;
Applications
ELISA, WB, IHC
Catalog Number
MB-011

Product Label

 

Description
Sepharose™
Color
n/a
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry