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Primary Antibodies  >  Cancer Research Antibodies
 

IDO2 Antibody

Rabbit Polyclonal
Code

600-401-C69
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Immunofluorescence of Anti-IDO2 Antibody.
Tissue: human IDO2 expressing HEK293 cells.
Fixation: 0.5% PFA.
Antigen retrieval:  not required.
Primary: IDO2 antibody at 1:50-1:1000 for 1 h at RT.
Secondary: Peroxidase rabbit secondary antibody at 1:10,000 for 45min at RT.
Localization:  IDO-2 is located in the cytosol.
Staining:  IDO-2 as green fluorescent signal with bis-benzimide nuclear counterstain (blue).
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Synonyms: Indoleamine 2,3-dioxygenase 2, INDOL1, Indoleamine-pyrrole 2,3-dioxygenase-like protein 1, IDO 2, IDO-2

IDO2 Antibody Properties

Description
Anti-IDO2 (RABBIT) Antibody - 600-401-C69
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse
Clonality
Polyclonal
Application
ELISA : 1:500-1:100,000
IF Microscopy : 1:50-1:1000
Western Blot : 1:2,000-1:10,000
ImmunoPrecipitation: 1:50-1:1000
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
100ug/ml by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.1% (w/v) Sodium Azide
GeneName
IDO2
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Lot#  

IDO2 Antibody Description

Background
IDO2 antibody recognizes Indoleamine 2,3-dioxygenase 2 (IDO2). IDO2 is related to IDO1, and may play a role in T and B cell immunity. IDO2 is expressed in a few select cell types and levels of protein expression appear to be very low, perhaps below the level of detection by IHC. It is detected in liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, and colon. IDO2 catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism. Activity of IDO2 is inhibited by 1MT (1-methyl-tryptophan) and MTH-trp (methylthiohydantoin-DL-tryptophan). D-1mT (1-methyl-D-tryptophan) may be an indirect inhibitor of the IDO2 pathway. Small-molecule inhibitors of indoleamine 2,3-dioxygenase (IDO) are currently being translated to clinic for evaluation as cancer therapeutics.
Immunogen
IDO2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a mixture of human and mouse full length protein corresponding to IDO2.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-IDO2 antibody has been tested for use in ELISA, IF, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 45.4 kDa in size corresponding to IDO2 protein by western blotting in the appropriate cell lysate or extract. IHC testing has been negative.
Purity/Specifity
Anti-IDO2 antibody is directed against IDO2 protein. The rabbit polyclonal antibody was affinity purified against the antigen. Prior to affinity purification the antibody was pre-cleared of any cross reactive antibody to IDO1 (human and mouse). A BLAST analysis was used to suggest cross-reactivity with IDO2 protein from human and mouse 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
Metz, R., DuHadaway, J., Rust, S., Muller, A. and Prendergast, G. (2010) The Antitumor Properties of Zinc Protoporphyrin IX, a Heme Oxygenase-1 Inhibitor, Is Dependent in Part on Functional IDO1. MolCancer Ther. Jun; 9(6):1864-71.
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302


Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, IP
Reacts With
human, mouse
Catalog Number
200-301-E57

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry