House TrueBlot GOC
 
You may also want to consider these related products.
Anti-MYC EPITOPE TAG (RABBIT) Antibody - 600-401-381
Anti-MYC EPITOPE TAG (RABBIT) Antibody - 600-401-381
Anti-MOUSE IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 610-1302
Anti-MOUSE IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 610-1302
BOVINE SERUM ALBUMIN 30% Solution - BSA-30
BOVINE SERUM ALBUMIN 30% Solution - BSA-30
Blocking Buffer for Fluorescent Western Blotting - MB-070
Blocking Buffer for Fluorescent Western Blotting - MB-070
Primary Antibodies  >  Cell Cycle Protein Antibodies
 

BIN1 Antibody

Mouse Monoclonal 99D IgG2b
Code

200-301-E60
Size

100 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Western Blot of Anti-BIN1 Antibody.
Lane 1:  Keratinocyte derived from Bin1 wild type mice.
Lane 2:  Keratinocyte derived from Bin1 null mice.
Load:  35 µg per lane.
Primary antibody: BIN1 monoclonal Antibody.
Secondary antibody:  IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT.
Block: 1xPBS, 0.4% Tween-20.
Other band(s): non-specific.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: AMPHL, Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, BIN-1, BIN 1, BIN1 antibody

BIN1 Antibody Properties

Description
Anti-BIN1 (MOUSE) Monoclonal Antibody - 200-301-E60
Host
Mouse
Target Species
Human
Known Cross Reactivity
mouse, human
Clonality
Monoclonal 99D IgG2b
Application
ELISA : 1:5000-1:50000
FlowCytometry : 0.5-1x10^6 cells
Western Blot : 1:500-1:1500
Immunochemistry: 1:100-1:500
ImmunoPrecipitation: 10-100 µL
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG2b
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
BIN1
Download CofA
Lot#  

BIN1 Antibody Description

Background
Bin1 is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, actin organization, programmed cell death, stress responses, and transcriptional control. The first mammalian BAR protein to be discovered, Amphiphysin I (AmphI), was identified in an immunoscreen for proteins associated with the plasma membranes of synaptic neurons, functions in the control of clathrin-dependent synaptic vesicle endocytosis. The mammalian Bin1 gene was first identified in a two hybrid screen for polypeptides that bind to the N-terminal Myc box 1 (MB1) portion of the c-Myc oncoprotein. Bin1 is similar to AmphI in overall structure, with an N-terminal BAR domain and a C-terminal SH3 domain. However, the Bin1 gene is more complex than the AmphI gene, encoding at least seven different splice variants that differ widely in subcellular localization, tissue distribution, and ascribed functions. Alternate splicing of the Bin1 gene results in ten transcript variants encoding different isoform. Bin1 is expressed ubiquitously in mammalian cells. Certain splice variants of Bin1 are expressed in the neurons, muscle cells or tumor cells. Bin1 may act as a cancer suppressor and inhibits malignant cell transformation. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Bin1 has also been implicated in Alzheimer disease and cardiac disease. Defects in Bin1 are the cause of centronuclear myopathy autosomal recessive; also known as autosomal recessive myotubular myopathy.
Immunogen
Anti-BIN1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with a fragment portion of recombinant human BIN1 protein followed by hybridoma development.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-BIN1 antibody has been tested for use in ELISA, Western Blot, IP, IHC, and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-BIN1 was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above. BIN1 antibody is specific for human BIN1 protein. A BLAST analysis was used to suggest cross-reactivity with BIN1 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with BIN1 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Nicot A. S. et al. (2007) Nat Genet. 39:1134-1139. Wechsler-Reya, R., D. et al. (1997) J. Biol Chem 272:31453-31458. Hong T. et al. (2010) PloS Biology 8:e1000312. Joseph H. L. et al. (2011) Arch Neurol. 69:320-328. Muller A. J. et al. (2003) Mol Cell Biol. 23:4295-306. Prendergast G. C. et al. (2009) Biochem. Biophys. Acta. 1795:25-36. Tsutsui, K. et al. (1997) Biochem. Biophys. Res. Commun. 236:178-183. Ramjaun, A. R. et al. (1997) J. Biol. Chem. 272:16700-16706. Butler, M. H. J. et al. (1997) Cell Biol. 137:1355-1367. Sakamuro, D. et al. (1996) Nat. Genet. 14:69-77. Lichte, B. R. et al. (1992) EMBO J. 11:2521-2530.
Specific Reference
Log in to submit your product review.

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Catalog Number
600-401-381

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry