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Primary Antibodies  >  Cell Cycle Protein Antibodies
 

BIN1 Antibody

Mouse Monoclonal 12A IgG1
Code

209-301-E62
Size

100 µg
Price

$309.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Western Blot of Mouse Anti-BIN-1 Antibody.  
Lane 1: LNCap.  
Lane 2: WM1341D. 
Lane 3: WM164. 
Lane 4: WM983A. 
Lane 5: WM793. 
Lane 6: WM239A. 
Lane 7: WM35. 
Load: 35 µg per lane.
Primary antibody: BIN-1 antibody (Exon 12A specific) at 1:400 for overnight at 4°C.
Secondary antibody:  IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: AMPHL, Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, BIN 1, BIN-1, BIN1 antibody, anti-BIN1 antibody

BIN1 Antibody Properties

Description
Anti-BIN1 (MOUSE) Monoclonal Antibody - 209-301-E62
Host
Mouse
Target Species
Human
Known Cross Reactivity
human
Clonality
Monoclonal 12A IgG1
Application
ELISA : 1:5000-1:50000
Western Blot : 1:500-1:1500
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG1
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
BIN1
Related Links
Download CofA
Lot#  

BIN1 Antibody Description

Background
Bin1 is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, actin organization, programmed cell death, stress responses, and transcriptional control. The first mammalian BAR protein to be discovered, Amphiphysin I (AmphI), was identified in an immunoscreen for proteins associated with the plasma membranes of synaptic neurons, functions in the control of clathrin-dependent synaptic vesicle endocytosis. The mammalian Bin1 gene was first identified in a two hybrid screen for polypeptides that bind to the N-terminal Myc box 1 (MB1) portion of the c-Myc oncoprotein. Bin1 is similar to AmphI in overall structure, with an N-terminal BAR domain and a C-terminal SH3 domain. However, the Bin1 gene is more complex than the AmphI gene, encoding at least seven different splice variants that differ widely in subcellular localization, tissue distribution, and ascribed functions. Alternate splicing of the Bin1 gene results in ten transcript variants encoding different isoform. Bin1 is expressed ubiquitously in mammalian cells. Certain splice variants of Bin1 are expressed in the neurons, muscle cells or tumor cells. Bin1 may act with cancer suppressor and inhibits malignant cell transformation. A Study in human tumor cell lines found that most melanoma cells inappropriately expressed exon 12A, suggests that the aberrant splicing of Bin1 may contribute to melanoma progression.
Immunogen
Anti-BIN1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with 12A exon BIN1 protein followed by hybridoma development.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-BIN1 antibody has been tested for use in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-BIN1 was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human BIN1 protein. A BLAST analysis was used to suggest cross-reactivity with BIN1 from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with BIN1 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Wechsler-Reya, R., D. et al. (1997) J. Biol Chem 272:31453-31458. Prendergast G. C. et al. (2009) Biochem. Biophys. Acta. 1795:25-36. Tsutsui, K. et al. (1997) Biochem. Biophys. Res. Commun. 236:178-183. Ramjaun, A. R. et al. (1997) J. Biol. Chem. 272:16700-16706. Butler, M. H. J. et al. (1997) Cell Biol. 137:1355-1367. Sakamuro, D. et al. (1996) Nat. Genet. 14:69-77. Lichte, B. R. et al. (1992) EMBO J. 11:2521-2530. Ge K., et al. (1999) Pro Natl Acad Sci. 96:9689-9694.
Specific Reference
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Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Catalog Number
600-401-381

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry