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IDO2 Antibody

Mouse Monoclonal 1HC IgG
NCI Collaboration
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Western Blot of Mouse Anti-IDO2 antibody.  
Lane 1: HEK293 no transgene cell extracts.
Lane 2: mouse IDO-2 transgene expressed in 293HEK.
Lane 3: mouse IDO-1 transgene expressed in 293HEK.
Primary antibody:  IDO2 monoclonal Antibody.
Secondary antibody:  IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT.
Block:  5% BLOTTO overnight at 4°C.
Predicted/Observed size:  44.4 kDa, ~60 kDa for IDO 2. 
Other band(s): IDO2 splice variants and isoforms.
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Number:
210-301-E59
Size:
100 µg
Price:
$309.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

IDO2 Antibody Properties

Description
Anti-IDO2 (MOUSE) Monoclonal Antibody - 210-301-E59
Host
Mouse
Target Species
Mouse
Known Cross Reactivity
mouse
Clonality
Monoclonal 1HC IgG
Application
ELISA : 1:5000-1:50000
IF Microscopy : 1:50-1:100
Western Blot : 1:500-1:1500
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
Ido2
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IDO2 Antibody Description

Background
Indoleamine 2,3-dioxygenase 2 (IDO2) is related to IDO1, and may play a role in T and B Cell Immunity. IDO2’s role in immune regulation will be become clearer following the completion of genetic knock-out studies. IDO2 is expressed in a few select cell types and levels of protein expression appear to be very low, perhaps below the level of detection by IHC. Small-molecule inhibitors of indoleamine 2,3-dioxygenase (IDO) are currently being translated to clinic for evaluation as cancer therapeutics.
Synonyms
Indoleamine 2,3-dioxygenase 2, Indol1, Indoleamine 2,3-dioxygenase-like protein 1, Indoleamine-pyrrole 2,3-dioxygenase-like protein 1, Ido-2, Ido 2, IDO2 antibody, anti-IDO2 antibody
Immunogen
Anti-IDO2 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with complete mouse IDO2 protein followed by hybridoma development.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-IDO2 antibody has been tested for use in ELISA, Western Blot, and IF. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-IDO2 was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. IDO2 antibody is specific for mouse IDO2 protein. Cross-reactivity with IDO2 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
Metz R., DuHadaway J., Rust S., Muller A., and Prendergast G. (2010) The Antitumor Properties of Zinc Protoporphyrin IX, a Heme Oxygenase-1 Inhibitor, Is Dependant in Part on Functional IDO1. MolCancer Ther. Jun; 9(6):1864-71.
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Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, IP
Reacts With
human, mouse
Catalog Number
200-301-E57

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry