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Primary Antibodies  >  Antibodies for Signal Enhancement
 

KLH Antibody

Mouse Monoclonal 3B1 IgM
Code

200-301-E66
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Keyhole Limpet Hemocyanin, KLH antibody, anti-KLH antibody

KLH Antibody Properties

Description
Anti-KLH (MOUSE) Monoclonal Antibody - 200-301-E66
Host
Mouse
Known Cross Reactivity
KLH
Clonality
Monoclonal 3B1 IgM
Application
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunohistochemistry: 1:1,000 - 1:5,000
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgM
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Preservative
0.1% (w/v) Sodium Azide
Stabilizer
None
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Lot#  

KLH Antibody Description

Background
KLH antibody is used to detect Keyhole Limpet Hemocyanin. KLH is a copper-containing oxygen carrier occurring freely dissolved in the hemolymph of many mollusks and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.
Immunogen
Anti-KLH (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with KLH (Keyhole Limpet Hemocyanin) followed by hybridoma development.
Immunogen Type
Native Protein
Storage Condition
Store KLH antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-KLH can be used as an IgM isotype control in various assays or in ELISA assays to detect KLH or KLH conjugates. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-KLH has been prepared from mouse ascitic fluid by immunoaffinity chromatography. KLH antibody is specific for KLH. Does not cross react with mouse heart or skin tissue sections. Cross-reactivity from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Reacts With
KLH
Catalog Number
200-301-466

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC
Catalog Number
600-401-466

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-1302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry