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Primary Antibodies  >  Sample Size Primary Antibodies
 

beta Actin Antibody

Mouse Monoclonal 3H2.E4.E12 IgG2a lambda
Code

200-301-E69S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
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Immunohistochemistry of Mouse anti-Beta Actin N-Term antibody.  
Tissue:  human spleen. 
Fixation:  formalin fixed paraffin archival tissues.  
Antigen retrieval:  not required.
Primary antibody:  Beta Actin N-Term antibody  at 2.5 µg/mL  for 1 h at RT.
Secondary antibody:  Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT.
Localization:  membranous and cytoplasmic.
Staining:  antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
Western Blot of Mouse anti-N-Term Beta Actin Monoclonal Antibody.  
Lane 1:  HeLa Whole Cell Lysate.
Lane 2:  NIH/3T3 Whole Cell Lysate.
Load:  10 µg per lane.
Primary antibody:  N-Term Beta Actin Monoclonal Antibody at 1.13 ug/mL overnight at 4°C.
Secondary antibody:  HRP mouse secondary antibody at 1:40,000 for 30 min at RT.
Block:  MB-070 for 30 min at RT.
Predicted/Observed size:  42 kDa, 42 kDa for N-Term Beta Actin. 
Other band(s): None.
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Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

beta Actin Antibody Properties

Description
Anti-beta Actin (MOUSE) Monoclonal Antibody - 200-301-E69S
Host
Mouse
Target Species
Human
Known Cross Reactivity
human, mouse
Clonality
Monoclonal 3H2.E4.E12 IgG2a lambda
Application
ELISA : 1:5000-1:50000
Western Blot : 1 µg/mL
Immunochemistry: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG2a
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
Actb
Related Links
Download CofA
Lot#  

beta Actin Antibody Description

Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.
Immunogen
Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with ß-Actin peptide near the N-Terminus followed by hybridoma development.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
Anti-beta Actin antibody has been tested for use in ELISA, IHC and Western Blot. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-beta Actin was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above. beta Actin antibody is specific for human and mouse ß Actin protein. Expected to cross-react with a wide range of species due to sequence homology. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates. Cross-reactivity with other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
Mouse, Rat, Human, Monkey, Sheep, Bovine, Goat, Guinea Pig, Swine, Hamster
Catalog Number
600-401-886

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-30

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry